| BackgroundWith the economic and social development of our country the incidence of cardiovascular diseases has been ranked first in various diseases.Cardiovascular disease is now and in the future a long time human beings have to face the most significant public health problems.With the economic and social development of our country,the incidence of cardiovascular diseases has been ranked first in various diseases.Cardiovascular disease is now and in the future a long time the most significant public health problems which human beings have to face.In cardiovascular disease,atherosclerosis and its complications account for a considerable part. Atherosclerosis is undermining the destruction of vascular endothelial cells,the waver of macrophages,Hyperplasia in smooth muscle cells and fibroblasts,as well as the accumulation of extracellular lipid mainly of a pathological process.There are a variety of theories about the pathogenesis of Atherosclerosis,but the specific mechanism of AS have not yet been fully clarified.The relationship between lipid and coronary heart disease has been confirmed.A large number of epiderniological data show that the severity of Atherosclerosis was positively correlated with the plasma cholesterol level.The increasion of levels in Low-density lipoprotein cholesterol are the most important risk factors for atherosclerosis,and cardiovascular and cerebrovascular diseases caused by atherosclerosis.In recent years,studies have proved that normal LDL can not be effectively stimulated macrophages into foam cells,and only after modification, mainly in a state of oxidative stress,was oxidized to Ox-LDL can stimulate macrophages into foam cells soon,which is conducive to atherosclerosis happen.In Oxidative stress status,the vessel wall-generated excess ROS can oxidize LDL to Ox-LDL,which is atherosclerotic pathology of the most important early event. Therefore,oxidative stress and oxidized low density lipoprotein at atherosclerosis plays an important role.Therefore,antioxidant therapy becomes the key aspect in blocking the process of atherosclerosis.Currently used for clinical main natural antioxidants VitC,VitE andβ-carotene. VitC is water-soluble antioxidants,it is not easy to bind with the lipid-bound and thus exert anti-lipid peroxidation,VitE andβ-carotene as a fat-soluble antioxidants,free radicals and oxygen for its reversible binding,antioxidant power is relatively weak. To do this urgently looking for a better antioxidant.LA,belong to vitamin B compounds,belong to a metabolic antioxidant,natural antioxidants,are known to one of the strongest performing.A large number of experiments have proved that LA has more than VitC and VitE powerful antioxidant.In recent years,LA for its powerful antioxidant and its application in diabetes and the complications of diabetes,as well as ischemia-reperfusion injury in such areas as the treatment of much of international bio-medical industry.To ascertain the pathway of the role of sustained-release alpha-lipoic acid tablet in preventing experimental atherosclerosis utilizing rabbit dietary atherosclerosis models.It could provide a theoretical basis,as well as sulfur acid sustained-release tablets provide experimental basis for clinical application. Objective1.To observe the effects of lipoic acid on lipid;2.To observe the effects of lipoic acid on the effects of oxidative stress;3.To observe the effects of lipoic acid on oxidized low-density lipoprotein levels;4.To observe the effects of lipoic acid on atherosclerosis intervention effect;5.To explore the intervention mechanism of lipoic acid on atherosclerosis.Methods1.Subjects:Select 24 healthy New Zealand white rabbits,male and female informal,body mass(2.3-2.7)kg,fed after 1 week were randomly divided into 3 groups,respectively, the normal group,model group,lipoic acid groups of eight.2.Methods:Ordinary particles to give one weeks after feeding,24 rabbits were randomly divided into 3 groups,normal group,model group,lipoic acid group.①Normal group:fed with ordinary feed particles.②model group:fed with high fat diet,that is,in ordinary feed add 1% cholesterol and 8%of lard.③lipoic acid groups:with high-fat diet,another given to each rabbit through the gastric tube lipoic acid 300 mg / d.Feeding high fat diet and preventive medication for 12 weeks,the experiment a total of 13 weeks.Ear fasting venous blood 12mL,rabbit serum triglyceride,total cholesterol, high density lipoprotein cholesterol,low density lipoprotein cholesterol levels in the zhujiang hospital clinical laboratory by automatic biochemistry analyzer finish. Rabbit serum malondialdehyde,superoxide dismutase,oxidized low-density lipoprotein,endothelin-1 and nitric oxide levels with reference to detection kit instructions.3.Statistical methods:All information used SPSS13.0 statistical software for statistical analysis. Normal data use descriptive statistical analysis of mean±standard deviation((?)±s), statistical inference used mainly one-way ANOVA,both the number of 22 compared the use of LSD-t test;skewed distribution of data into normal distribution after the treatment,such as the transformation of the way without proper while the use of non-parametric test,comparing the two groups using Wilcoxon rank sum test,many groups using Kruskal-Wallis H test.Serum malondialdehyde,superoxide dismutase, oxidized low-density lipoprotein,endothelin and nitric oxide in the correlation between the level of simple correlation analysis.In this paper,all statistical inference testing standards set toα=0.05.Results1.A quantitative analysis of experimental animals.All the 24 rabbits were included in the final analysis,without drop-outs.2.After the rabbits were fed with high fat forage,the levels of serum total cholesterol,triglyceride,low density lipoprotein cholesterol in the model group were significantly higher than in the normal group(P＜0.05),but high density lipoprotein cholesterol was significantly lower than in the normal group(P＜0.05).The levels of serum total cholesterol,triglyceride,low density lipoprotein cholesterol in the lipoic acid group were significantly lower than in the model group(P＜0.05).High-density lipoprotein cholesterol levels compared with the model group the difference was not significant(P＞0.05). 3.The level of serum nitric oxide and superoxide dismutase in the Model group were significantly lower than the control group(P＜0.05),The level of serum malondialdehyde,endothelin-1 and oxidized low density lipoprotein were significantly higher than the normal control group(P＜0.05 );The level of serum of nitric oxide and superoxide dismutase in lipoic acid group were significantly higher than the model group(P＜0.05),The level of serum malondialdehyde,endothelin and the level of oxidized low density lipoprotein was significantly lower than model group(P＜0.05 ).4.The level of oxidized low density lipoprotein and the level of superoxide dismutase into a significant negative correlation(r=-0.862,P=0.000);oxidized low-density lipoprotein levels and malondialdehyde levels into a significant positive correlation(r=0.946,P = 0.000);the level of oxidized low density lipoprotein levels and nitric oxide into a significant negative correlation(r=-0.906,P = 0.000);oxidized low-density lipoprotein level and the level of endothelin into a significant positive correlation(r = 0.894,P = 0.000).5.When LDL and MDA level as the covariate,the dependent variable as a grouping to Ox-LDL for the response variables,We analysis the covariance:at the exclusion of LDL,MDA impact,and find that Lipoic acid can significantly reduce the Ox-LDL levels(F = 8.564,P = 0.002).ConclusionOur data indicate that:1.Lipoic acid in rabbits with hyperlipidemia can reduce the level of TC,TG,LDL;2.Lipoic acid can relieve the level of oxidative stress;3.Lipoic acid can reduce hyperlipidemia rabbit serum oxidized low-density lipoprotein level.4.Lipoic acid has the role of vascular endothelial protection and Antiatherosclerosis. To sum up,self-lipoic acid sustained release tablets in the body through reducing lipid levels,removal of excessive superoxide anion and other free radicals, reducing lipid peroxidation and reduce the generation of Ox-LDL,the protection of vascular endothelial and the improvation of vascular endothelial dysfunction, eventually plays the role of atherosclerosis prevention. |
PDF Full Text Request