Preparation And Improvement Of Nanophase Artificial Bone Composite Scaffold And Relevant Study On Its Properties

Objective Prepare nanophase artificial bone composite scaffold basing on bionics theory,and probe into how different content ratio between collagen and inorganic part as well as different molecular weight of conglutinant agent influence microstructure and property of the scaffold.Method Lead calcic inorganic molecules to deposite onto self-assembled collagen template during coprecipitation under certain reactive condition such as content ratio and PH value of environment, and then nanophase collagen/calcic salt is obtained.In order to improve the mechanical properties,poly lactic acid(PLLA) which has stable equal properties and controllable biodegradable activities is chosen as conglutinant agent,and then the aimed artificial bone scaffold(nanophase collagen/calcic salt/PLLA composite) is accomplished.After preparation liquid displacement method with isoproanol alcohol, sacnning electron microscopic(SEM),transmission electron microscope(TEM), fourier transform infrared spectrometry(FT-IR),mechanical testing system are performed to test porosity and density,morphology,conformation,composition and mechanical property,respectively.Results The artificial bone composite scaffold synthesized is mainly composed of collagen and calcium phosphate partly displaced with B-type carbonate.The crystallinity is low and the crystal size is small enough to reach nanometer which is similar to natural bone.PLLA used effectively improves mechanical property which can reach the floor level of cancellous standard,and develops three-dimensional-porous structure with high porosity of 80%.According to comparison it can be seen that content ratio between collagen and calcic inorganic salt as well as addition of conglutinant PLLA all have effect on microstructure of the scaffold synthesized,additionally molecular weight of PLLA has effect on mechanical property.Conclusion The nanophase artificial bone composite scaffold synthesized by boimimetic process is one of the most promising optimal material for clinical application no matter judged from structure,composition and property.The various factors influencing the scaffold discussed in this article may indicate some useful modification ways according to actual utility in the near future.