Empirical Study Of The Effect Of Surgical Delay On The Ability Of Anti-infection Of The Reverse Fascio-cutaneous Flap

Objective:1.To observe the survival ability of the reverse fascio-cutaneous flap and its ability of anti-infection of different density liquid of Staphylococcus aureus.2. To observe the effect of the surgical delay on enhancing the ability of anti-infection of the reverse fascio-cutaneous flap.3.To observe the changing of the vascular diameter of the fascio-cutaneous flap.Method:The model of reverse fascio-cutaneous flap about 2cm×4cm based on sural nerve on the lateral side of hind limb lower leg was designed in the rabbits.To compare survival circumstance of the flap of raising different density liquid of Staphylococcus aureus and of delaying and non-delaying group of raising same density liquid of Staphylococcus aureus.To observe the changing of the vascular of fascio-cutaneous flap by color doppler ultrasound.To qualitative and quantitative analyse of the flap by white blood cell count,measuring necrosis area of the flap and bacterial culturing of necrosis tissue.To compare survival circumstance of the flap by measuring the area of survival.Results:The survival area of raising different density liquid of Staphylococcus aureus is different(P＜0.05).The survival area,white blood count,the caliber and bloodstream velocity of the vascellum diameter under flap of delaying group of raising same density liquid of Staphylococcus aureus is obviously better than that of non-delaying group(P＜0.05).Conclusion:The survival area of the reverse fascio-cutaneous flap,the vascular diameter and bloodstream velocity of the vasculum under flap increasing obviously by surgical delay and it has the ability of enhancing survival and anti-infection of the reverse ascio-cutaneous flap.