The Effects Of Acute Rhinosinusitis On The Regeneration Of Olfactory Receptor Neurons

The Establishment of a rat model of acute rhinosinusitisObjective:To develop a rat model of acute rhinosinusitis,providing basis for the experiments afterwards.Methods:Fifty healthy rats were randomly divided into two groups:model group(forty rats),and the control group(ten rats).Rats in the model group were inoculated with Streptococcus pneumoniae with the help of Merocel;and the rats in the control group were inoculated with sterilized saline.All the rats in the control group were sacrificed in one week after the inoculation,and the rats in the other group were sacrificed in one week,two weeks,three weeks and four weeks after the inoculation respectively,ten rats each time. The issues containing nasal cavity and sinuses were obtained immediately, and were treated according to the HE procedures.Optical microscope was used to observe the results.Results:There was no pathological change in the mucous membrane of the rats in the control group,but in that of the other group,the inflammatory reaction began to appear in the first week of the inoculation of Streptococcus pneumoniae,and it became more severe in the second week.But the inflammatory reaction decreased since the third week,and in the fourth week,the mucous membrane almost completely recovered to normal.Conclusions:With the help of Merocel, the inoculation of Streptococcus pneumoniae intranasally can induce acute rhinosinusitis in rats.The detection of olfactory function of the ratsObjective:To detect the olfactory function of the rats,and analyze whether there was some kind of changes in terras of the olfaction between each group of the rats,and what the changes would be.Methods:On the basis of the first part of the experiment,a method called "buried food pullet" was used to measure the olfaction of the rats,and the time of every rat spending in finding out the food pullet was recorded and analyzed. Results:Compared with the rats in the control group,all the rats inoculated with Streptococcuspneumoniae spent more time in searching the food pullet,with the fourth-week group the longest,and the second-week the shortest comparatively.And the difference between them was significant(P＜0.01).Conclusions:In comparison with the rats in the control group,all the rats inoculated with Streptococcus pneumoniae got inferior olfactory function.And relatively speaking,the olfaction of the rats decreased to the lowest point two weeks after the inoculation of the Streptococcus pneumoniae,and it recovered a little from then on.The effects of acute rhinosinusitis on the regeneration of the olfactory receptor neuronsObjective:To observe the regeneration of olfactory receptor neurons (ORNs) in the acute rhinosinusitis model,providing foundation for further exploration of corresponding mechanism.Methods:On the basis of the first part of the experiment,the method of double labelling was employed to display the changes of ORNs' regeneration in the olfactory sensory epithelium(OE).All the ten rats in the control group were sacrificed in one week,two weeks,three weeks,and four weeks after the inoculation respectively,ten rats each time.The olfactory mucous membrane was dissected from the nasal septum,and was handled according to the procedures of cryosections step by step.Slices were double labeled by antibody of NSE(marker of neurons) and antibody of BrdU(marker of new cells).The cells that were double labled by the antibody of NSE and that of BrdU would be new ORNs.Results:There were sparse new ORNs in the OE of the control rats,and the new ORNs increased in the rats inoculated with Streptococcus pneumoniae.The phenomenon of regenetation was much more obvious in the third and fourth week than that in the first and second week.Conclusions:Acute rhinosinusitis can induce the regeneration of ORNs,and the rate of regeneration in the late phase of the inflammatory reaction was faster than that in the early phase.