The Effect Of Qiguiyimailing On Acute Myocardial Ischemia Reperfusion In Rat Cardiac Myocyte Apoptosis Genes Expression

Objective:To observe the effect of QiGuiYiMaiLing(QGYML) on acute myocardial ischemic -reperfusion in rat cardiac myocyte apoptosis related genes Fas,Bcl-2 protein expression to explore their effects and mechanism of myocardial protection.Methods:The left coronary artery ligation were prepared threading myocardial ischemic -reper -fusion model,Wistar rats with 70,half male and female,were randomly divided into seven groups(n=10) and ID:blank control group,sham-operated group,ischemia- reperfusion group,ischemic preconditioning group,isosorbide dinitrate group,low-dose QGYML and QGYML high-dose group,We observe the morphological changes of apoptotic cells by HE staining in electron microscopy and check cardiomyocyte apoptosis related genes Fas, Bcl-2 protein positive expression by immunohistochemical staining.Results:1.In addition to the sham-operated group and blank control group,the remaining myocytes in each group has a different degree of morphological changes.Especially, ischemic -reperfusion injury group was significant for the cell morphology.2.QGYML high-and low-dose group,isosorbide dinitrate group,ischemic preconditioning group increased significantly than ischemic-reperfusion group Bcl-2 protein expression(P＜0.05);Effects of QGYML high-dose group may significantly increase the expression of Bcl-2 protein,and compared with low-dose group,the result is significantly different(P＜0.05),and compared with isosorbide dinitrate group,there was no significant difference(P＞0.05).3.Ischemic-reperfusion group Fas protein expression is the highest,compared with the other groups,and significantly different(P＜0.05),QGYML significantly reduced its expression,and high-dose group was significant,compared with isosorbide dinitrate group and ischemic preconditioning group,the result is equivalent comparison and among the three were no significant differences(P＞0.05).Conclusion: 1.QGYML can reduce the acute myocardial ischemic-reperfusion rat model of myocardial structural abnormalities.2.QGYML,through reduced expression of Fas gene protein and increased Bcl-2 gene protein expression,inhibited cardiomyocyte apoptosis and has a certain role in anti-myoca rdial ischemia.3.The role of QGYML gene regulation of apoptosis is similar to ischemic preconditioning group and isosorbide dinitrate group,may have its pharmacological preconditioning -like myocardial protection.