| ObjectiveMucociliary transport system in airway mucosa is most important nature defense system remained in the body after endotracheal intubation,which is able to clear pathological granules and microorganism.Cilia beat frequency(CBF)play a very important role in mucociliary transport system.It is known that volatile anesthetics inhibit mucociliary transport function of airway mucosa.Total intraveneous anesthesia of Remifentanil has been widely used in clinic.But the study about the effects of Remifentanil on mucociliary transport velocity and cilia beat frequency of airway epithelium is not noted commonly.We studied the effects of Remifentanil on the bronchial mucociliary transport velocity and investigated the effects of Remifentanil on cilia beat frequency in order to explain the action of Rimifentanil on airway mucosa transport function.MethodsThe palate was sampled from 30 matured toads.The mucosa of palates was cut into 2 mm×2 mm small pieces under the stereoscope and then put them into control group and intervention group.Control group:0.9%normal saline.Intervention group with Remifentanil which is diluted into three concentrations;1ng/ml,4ng/ml and 16ng/ml.The small pieces were divided into four groups and randomly dipped into different solutions mentioned above.Actively beating ciliated cells were observed and their motion was quantified by measuring CBF using phase-contrast microscopy.CBF was counted manually at baseline 5,30,and 60 min respectively.Results1.The CBF in 1ng/ml group had no significance at any point of time compared with that of control group(p＞0.05).2.The CBF in 4ng/ml group decreased significantly at the point of 5 and 30 min compared with that of control group(p＜0.05).But the CBF in 4ng/ml group had no significance at the point of 60 min(p＞0.05).3.The CBF in 16ng/ml decreased significantly at the point of 5 and 30 min(p＜0.05),but which had no significance at other points of time(p＞0.05).4.At the point of 5 min,CBF of the interventional groups decreased significantly,and the the level of the CBF was down with the increase of Remifentanil's concentration.ConclusionMucociliary transport system(MTS) is an important defence against respiratory tract infections.The depressant effect of inhalation anaesthetic agents on mucus transport in vivo are well established in both animals and humans.Cilia beat frequency (CBF)is an important determinant of the mucus transport rate(MTR);there is a logarithmic relation between the two such that modest reduction in cilia beat frequency(CBF) are associated with substantial reductiona in mucus transport rate(MTR).In this study,we utilized the frog palate as a model to assess functional injuries to the mucociliary epithelium.The choice of using the mucociliary system(MS) as the toxicological end-point is logical,since it represents the main defense of the lining epithelial layer of the respiratory system,which is the natural interface between the organism and the environment.The epithelium of the frog palate is similar to the airway epithelium of mammals in many aspects,permitting shor-term investigations of the effects of substances on ciliary properties such as beat frequency.Remifentanil decrease CBF of isolated toad palate. |
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