Study On Preparation Of Lycopene Nanoliposomes, Absorption And Antioxidation In Vivo

Lycopene is a kind of carotenoids that can't be synthesized by body,it shows,power antioxidant.However the application of lycopene has been restricted which is attributed to the low aqueous solubility and oral bioavailability.Lycopene nanoliposomes were prepared by conventional rataryevaporated film-ultras onication.The encapsulating efficiency of lycopene nanoliposomes was measured by the centrifugal extraction-vis spectrophotometer.It was taken as the main criteria,the prescription of the lycopene nanoliposomes were optimized by orthogonal design.The data showed that the best ratio of cholesterol: phospholipids was 0.15:1,lycopene:phospholipids was 0.02:1,the optimum pH value of the water phase phosphate buffer solution was 7.0 and the temperature was 34℃The optimized operation of lycopene nanoliposomes were reasonable, practicable with low particle size with homogeneous microspheres shape,the particle size was below 40nm.The photographs of disrupted of membrane before and after confirmed that the lycopene was successfully encapsulated into the nanoliposomes materials.Infrared spectroscopy spectra(FTIR) and X-Ray Diffraction patterns(XRD) were used to characterize the structure of the nanoliposomes.The mice were fed with lycopene nanoliposomes and oil lycopene,the content of lycopene in liver and serum were determined by HPLC.The results showed that the content of lycopene in liver and serum in lycopene nanoliposomes group was higher than oil lycopene group,especially in nanoliposomes group the content of lycopene in liver was significantly higher than in oil lycopene group(p＜0.01),but in serun was no significantly change.The preliminary experiment indicated that lycopene was higher accumulation in the liver by passive targeting nanoliposomes as the delivery system,and the bioavailability was improved.The activities of SOD,POD,CAT,and the content of MDA were determined in liver.The results showed that both of the oil group and nanoliposomes group the activities of SOD,POD,CAT were significantly higher than in control group,and the concent of MDA decreased highly.Especially nanoliposomes group made outstanding contribution in anti-oxidative capability.These results further show that hydrophobic lycopene had effect on accumulation in the liver by passive targeting with nanoliposomes as product,and the bioefficacy and bioavailability was improved.This experiment provides a certain basis for further study lipophilic medicam-ent and preparation or production food nanoliposomes.