Selection Of Peptides That Bind The First Loop Of CCR5 (ECL-1) From A Phage-displayed Peptide Library And The Experimental Study On Anti-inflammatory Effects

Objective:To select peptides that bind the first loop of CCR5(ECL-1)from a Ph.D.-7^TM phage-displayed peptide library and observe the effects of the peptides on xylene-induced ear swelling in mice.Methods:ECL-1 was used as ligand to screen the binding peptide from the Ph.D.-7^TM phage-displayed peptide library the specificity of colons were identified by ELISA,the same peptide sequence was synthesized after DNA Sequencing.Through the mouse ear swelling and H＆E staining to observe the effects of the peptides on xylene-induced ear swelling in mice.Results:Through three rounds of biopanning,15 phage clones randomly selected were positive,and had the same inserted amino acid sequence.The peptide and the positive phage clones can reduce edema,hyperemia and infiltration of inflammatory cells obviously in xylene-induced ear swelling.Conclusion:A peptide and the positive phage clones with high affinity binding ECL-1 were obtained successfully by the phage display library,which can inhibit the exudation, edema,inflammatory cell infiltration of the early inflammatory.The mechanism of anti-inflammatory may be blocking the combination of CCR5 and its ligand,thereby inhibiting infiltration and migration of the leukocyte.In this study,a new molecular was provided as a candidate for the targeted therapy for inflammation.At the same time,this study had laid a solid foundation for the further identification and development of anti-inflammatory drugs.