| [Objective]To research the impact of the level of blood glucose by fasting, to set up an experimental hypoglycemia model,and observe the brain damaged of neonate rat with hypoglycemia model. To study the mechanism that hypoglycemia induce brain damaged, and to provide experimental data of feeding patterns and precaution of hypoglycemia in clinical.[Methods]150 neonate Wistar rats were randomly divided into 5 groups.Each group had 30 rats. Group A was control group; Group B,C,D and E were fasting group. Group B were fasted for 4 hours, Group C were fasted for 8 hours,Group D were fasted for 12 hours and Group E were fasted for 16 hours. Then we observe the level of blood glucose and the pathological change of occipital region cortex and the change of ethology of rat in Juvenile.[Results]1. The persistence time of fasting induces the differet level of blood glucose, and to set up the neonate rats with experimental hypoglycemia model well.The level of blood glucose in control group of neonate rat was normal by normal feeding. The level of blood glucose of the Group B was5.43±0.48mmol/L, comparing control group P>0.05, and there was no conspicuous descent. But the level of blood glucose of Group C,D and E were respectively4.21±0.42mmol/L,3.24±0.11mmol/L and2.76±0.26mmol/L,.Comparing control group P<0.05 , there was statistical significance in the level of blood glucose of Group B, GroupC,Group D,Group E.2. The saturation of blood oxygen before and after fastingThe saturation of blood oxygen of Group A,B,C,D and E were 54.3±2.35mmHg,52.7±1.13mmHg,53.7±1.82mmHg,51.9±2.10mmHg and 52.7±0.91mmHg after fasting, respectively. There was no statistical significance in blood gas analysis between before and after fasting (P>0.05). The experimental results of saturation of blood oxygen cued that the impact of the saturation of blood oxygen by fasting diet was light.3. The pathological change of occipital region cortex of neonate rat3.1 The pathological change of occipital region cortex under the light microscopeThere was rarely neuron injury in control group ,which nucleus of neuron was small and circular, and located in center of neuron, heterochromatin was rare and light blue, nucleolus located in nucleus clearly; endochylema was salmon pink. There was also rarely neuron injury in Group B, which nucleus of neuron was small and circular, and located in center of neuron, heterochromatin was rare and light blue, nucleolus located in nucleus clearly; endochylema was salmon pink. There was a small quantity neuron injury in Group C,which was edema change and the volume of neuron was bigger than normal neuron, which ucleus of neuron was bigger than control group and located in center of neuron, heterochromatin was rare and the staining was lighter; endochylema was rarefaction. There was more neuron injury in Group D ,which was edema change and the volume of neuron was bigger than normal neuron, which ucleus of neuron was bigger than control group and located in center of neuron, heterochromatin was rare and the staining was lighter; endochylema was rarefaction. There was a great quantity neuron injury in GroupE ,which was edema change and the volume of neuron was bigger than the neuron of Group C and Group D evidently, which ucleus of neuron was bigger and located in center of neuron, nucleolus was not clear in nucleus, there was vacuole in heterochromatini and the staining was lighter evidently. Some time vasodilatation was observated. The comparison percentage of normal neuron of occipital region cortex of neonate rat: The percentage of normal neuron Group A,B,C,D and E were 96.3±1.5%,92.6±2.1%,81.1±4.7%,69.0±3.8% and 22.6±9.7%, respectively. There was no statistical significance between control group and Group B (P>0.05), there was statistical significance between control group and Group C, Group D, Group E (P<0.05), there was statistical significance between Group C and Group D (P<0.05), there was statistical significance between Group D and Group E (P<0.05).3.2 The pathological change of occipital region cortex under electron microscopeControl group(Group A): The cell membrane of neuron was integrity, the construction of mitochondrion was normal, mitochondrial crista was well-arranged and ground substance was well-distributed, the shape of endoplasmic reticulum was normal, nuclear membrane of nucleus was normal, nucleolus located in nucleus, and heterochromatin was well-arranged.Group B: The cell membrane of neuron was integrity, the construction of mitochondrion was normal, mitochondrial crista was folding and ground substance was well-distributed, the shape of endoplasmic reticulum was normal, nuclear membrane of nucleus was normal, nucleolus located in nucleus, and heterochromatin was well-arranged.Group C: The cell membrane of neuron was integrity, nuclear membrane of nucleus was normal, nucleolus locate in nucleus, heterochromatin was well-arranged, the granulation of mitochondrion disappear and ground substance was transparence, mitochondrial crista was not disruption, and the shape of endoplasmic reticulum was dilat.Group D: The cell membrane of neuron was integrity, the granulation of mitochondrion disappear and ground substance was transparence generally, mitochondrial crista was disruption ,the shape of endoplasmic reticulum was dilat, the vesicle change was found. Concentrate, and the nucleolus located in nuclear membrane of nucleus.Group E: The cell membrane of neuron was not integrity, the granulation of mitochondrion disappear and ground substance was transparence generally, mitochondrial crista was disruption generally, mitochondrion was vacuolus change, the shape of endoplasmic reticulum is dilat, collapse and autoproteolysis, the vesicle change was found. concentrate; the nucleolus located in nuclear membrane of nucleus. 4. Ethology change(The change of potential of learning and memory)The frequency of train in water maze of the Group A,B,C,D and E were 8.44±1.02次,8.65±1.63次,8.48±1.23次,10.01±1.60次,11.98±2.31次, respectively. There was no statistical significance in the change of potential of learning and memory between Group B, Group C and control group (P>0.05); there is statistical significance in the change of potential of learning and memory between Group D, Group E and control group (P<0.05). The lower the level of blood glucose was, the more the frequency of train in water maze was.[Conclusion]1.Founding hypoglycemia model of neonate rats by fasting diet was reasonable.2.Not all hypoglycemia could induce brain damaged.3.The longer persistence time of fasting diet induce the lower level of blood glucose. Hypoglycaemia could induce brain injured,and the less the blood sugar level the more severe extent brain injured. |
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