Therapeutical Impact Of Potassium Chloride In Animals With Trimethyltin Chloride And The Effect On Expression Of Renal H-K-ATPase With Trimethyltin Chloride

Objects: To study the therapeutical effect of Potassium Chloride by oral and the effect of expression ofβ-subunit of renal H-K-ATPase by Trimethyltin Chloride. Method: (1) Rats, mice and rabbit were treated with TMT by peritoneal injection and by gavage to measure the acute toxicities (LD50) in these animals. Serum ions of potassium, sodium and chloride were also tested with Electrolyte analyzer (2)The 36 female SD rats were divided into 6 groups ,which were model group ,low, middle, high dosage Potassium Chloride therapeutical groups,pure Potassium Chloride group and blank group,using the randomization by weight.The 24-hour-metabolism test was started on the first day and the six day after expose the TMT. The Urine of animal were collected carefully to analyze the ion and PH.At the seventh day,the arterial blood were collected by heart to run blood gas analysis.The animals were executed by collecting the blood from the arteria cruralis and femoral vein,getting the plasma to measure ion and biochemical indicator.The kidney was selected to measure the renal H-K-ATPase and Na-K-ATPase activity,meanwhile,to preserve the tissure of the kidney in order to analyse the expression ofβ-subunit of renal H-K-ATPase by western blot and immunohistochemistry. The main organs of animal were selected to calculated the organ coefficient and examine the kidney pathematology.(3) The membrane protein of kidney was extracted by the plasma membrane protein extraction kit from the kidney tissure. The membrane protein were separated on 10% SDS-PAGE and fragment sizes were determined by western analysis with anti-β-subunit of renal H-K-ATPase antibodies.The destination protein bands were visualized by the enhanced chemiluminescence procedure and X-gel..Results: LD50 in the animals were 14.7 mg/kg(female and male rats, oral), 3.16mg/kg (female mice, ip), 4.64mg/kg (female mice, oral), 3.83mg/kg (male mice, ip), 3.16mg/kg (male mice, oral), 3.16 mg/kg(female rabbits, ip), 2.15mg/kg (female rabbits, oral), 2.61mg/kg(male rabbits, ip), and 3.16mg/kg (male rabbits, oral). Ion levels in serum indicated that potassium was significantly decreases, but sodium and chloride did not change obviously. After treated with TMT, the weight of animal and blood potassium were dropped,but the the volume of urine ,urine PH and urine potassium were step up. There are several blood and urine ion and biochemical indicator has changed such as AST,ALT,CK,CK-MB,LDH,HBDH. The HKA activity of model group was descented. The time of symptom appearance of KCl therapeutical groups were later than model group,and the HKA activity of these groups are higher than model group,but lower than blank group.The effect of Na-K-ATPase activity by TMT are not evidence. The effect of expression ofβ-subunit of renal H-K-ATPase by TMT are not significant. Conclusion : The TMT has different acute toxicity from various animals, and it can induce the hypokalemia in tested. The KCl can in antagonism to the effect of TMT inhibited the renal H-K-ATPase activity. The effect of the expression ofβ-subunit of renal H-K-ATPase by exposing TMT in 6 days was not observed.