The Experimental Study Of The Effect Of Rhodiola Herb Against Activity Of AchE,NOS And The Expression Bcl-2,Bax Proteinin The Hippocampus Tissue Of Alzheimer's Disease In Rats

Objective:Hydergine of western medicine for the control,by detecting the expression of Nitric oxide synthase(NOS),acetylcholines-terase (AchE) in hippocampus tissue of rats and content of bcl-2,bax protein in the hippocampus tissue and determine the ability of learning and memory of rats by behavior experiment. Evaluating the ability of learning and memory that Rhodiola improved the situation of Alzheimer's disease in rats. Exploring the mechanism of action that Rhodiola protected the brain of Alzheimer's disease in rats. Provide the experimental basis for Rhodiola clinically.Methods:(1)Model: AD model was formed by adopting the D-gal+ALCL3+SCOP in health male SD rats.(2)Packet: Reference to the literature using random number table this model success were randomly divided into three groups, namely, model group, Chinese drug(Rhodiola herb) group, western medicine (Hydergine) group, setting up a blank group.(3) Delivery methods: For 30 days,Rhodiola group and Hydergine group were intragastrically given to rats by a dose of 5g/kg/d and 1mg/kg/d respectively. While the rats in model group and blank group were intragastically given to 5ml/kg/d saline.(4)Detect indicators: Used Morris water maze detect the ability of learning and memory of rats, Into place navigation and spatial search experiment . Measured brain NOS,AchE absorbance of brain tissue NOS,AchE activity was by spectrophotometry, calculates the brain to organize the NOS,AchE vigor. Immunohistochemical detection of rat hippocampal CA1 Bcl-2, Bax protein expression was observed.Results:(1) learning and memory ability Detected by Water maze: AD model may decrease cognitive ability(P < 0. 01) compared with the blank group, Rhodiola herb significant alleviated cognitive ability(P < 0. 01) compared with AD mode group. No difference between the treatment group significantly(P>0.05). (2) AchE detected: AD model can improve the activity of AchE compared with the blank group (P < 0. 05), treatment group can reduce the activity of AchE compared with AD mode group(P < 0. 05), but no difference between the treatment group significantly(P>0.05). (3) NOS detected: AD model can reduce the activity of NOS compared with the blank group (P < 0. 05), treatment group can improve the activity of NOS compared with AD mode group(P < 0. 05), but no difference between the treatment group significantly(P>0.05)(4) apotosis-related Proteins detected: Bcl-2 and Bax in the blank group hippocampal CA1 a small amount of expression. Compared with blank control group, each group bcl-2, bax expression were significantly enhanced (P <0.05). Model group, more significant changes in bax, the ratio of Bcl-2/ Bax in the hippocampus comes down (p<0.05),Treatment group ,more significant changes in bcl-2, the ratio of Bcl-2/ Bax rises(p<0.05). but no difference between the treatment group significantly(P>0.05)Conclusions:(1) Rhodiola can improve the AD model rats the cognitive skills, improve learning and memory score, suggesting that Rhodiola may have a role in AD prevention and treatment. (2) Rhodiola can inhibit AchE activity in the hippocampus and increase the level of Ach of AD rats, suggesting that anti-cholinergic neuron injury is one of the mechanisms that Rhodiola improves cognitive function in AD rats. (3) Rhodiola can enhance NOS activity and the level of NO in the hippocampus of AD rats, suggesting that promoting NOS upregulation is one of the mechanisms that Rhodiola improves cognitive function in AD rats. (4) Rhodiola herb can inhibit apoptosis by increasing the expression of Bcl-2 and reducing the expression of Bax protein in the hippocampus. Rhodiola herb may improve cognitive ability in AD rats.