Study On The Expression Of Angiotensin â…¡(ANG â…¡) And Its Receptor Subtype 1 (AT1R) In Pregnancy-induced Hypertension.

ObjectivesThe human placenta has been considered to possess a locally generated renin-angiotensin systerm (RAS),which plays a physiological role in regulation of uteroplacental blood circulation. The changes in the expression of such a placental RAS during pregnancy should be important for the pathophysiological aspects of pregnancy-induced hypertention. The present study is to investigate the expression of AT1R in placenta and level of serum AngII and cord blood AngII in preeclampsia patients and discuss its roles in pathegenesis of preeclampsia.MethodsSemi-quantitative RT-PCR was carried out to detect the level of AT1R mRNA in placental of 20 preeclampsia (study group) and 15 normotensive pregnancies (control group). ELISA was used to detect the peripheral venous blood level of AngII and cord blood AngII in control group (n=20) and study group (n=30).Results1,AT1R mRNA level of placental was significantly higher in preeclampsia than that in normal pregnancy. (1.05士0.05)vs (0.95士0.04) (P<0.05).2,The peripheral venous blood level of AngII in preeclampsia was significantly lower than that in normal pregnancy. (27.35士0.15) pg/ml vs (44.08士0.18) pg/ml (P<0.05).3,The cord blood level of AngII in preeclampsia was significantly higher than that in normal pregnancy. (87.16士0.26) pg/ml vs (64.92士0.24) pg/ml (P<0.05).4,The cord blood level of AngII was significantly higher than the peripheral venous blood level of it not only in preeclampsia but also in normal pregnancy.ConclusionsPreeclampsia has higher level of the peripheral venous blood AngII and cord blood AngII expression than normal pregnancy. The upregulation of AngII expression in cord blood than peripheral venous blood suggest that the placenta may be the major source of elevated AngII. The alteration of AT1R mRNA in placental and AngII in serum may contribute to the pathogenesis of preeclampsia. The placental RAS was associated with the morbidity of preeclampsia.