| The liver failure caused by severe hepatitis can threat patient's life because of the accumulation of toxin in the body caused by hepatic encephalopathy.Aromatic amino acid is one kind of the most important toxins leading to hepatic encephalopathy.It is an efficient clinic treatment of sever hepatitis to remove the excessive aromatic amino acids in blood through blood purification.Hemoperfusion and Molecular adsorbent recycling system are two main blood purification methods.Hemoperfusion has the problems of weak hemocompatibility and nonspecific adsorption;Molecular adsorbent recycling system overcomes these disadvantages,but simultaneously raises its price.In this paper,we synthesize a series of water-soluble adsorbents using polyethylenimine as matrix and couplingβ-cyclodextrin as functional group according to structure characteristics of aromatic amino acids,and then perform synthesis optimization.Experimental results indicate:(1) The optional condition is qualified as follows:the molar ratio of sodium periodate(1.95g) toβ-cyclodextrin(20g) is 1:1,and the oxidation time is 6 h,and the additive amount of vector is 20 g,reaction temperature 20℃;(2) In the dynamic adsorption experiment,the adsorption of PEI-β-CD to aromatic amino acids reaches homeostasis in lh,and the order of adsorption ratios to different aromatic amino acids in the same condition is L-Trp＞L-Phe＞L-Tyr;(3) In the plasma experiment,the adsorption of PEI-β-CD to aromatic amino acids reaches homeostasis in 2h,and the order of adsorption ratios to different aromatic amino acids in same condition is L-Trp＞L-Phe＞L-Tyr,and the removal ratio is lower than that in the dynamic adsorption experiment;(4) 400ml adsorbent with 20 g/L concentration has much higher unit adsorption quantity in 6h;(5) In the plasma experiment,the adsorption ratio of PEI-β-CD to aromatic amino acids is much higher and more specific,while the removal ratios of other 15 amino acids are all below 5%. |
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