Protective Effect Of Scutellarin Against Vascular Endothelial Cell Injury Induced By Oxidative Damage And Its Molecular Mechanism

Objective:To investigate the protective effects of scutellarin on vascular endothelial cell injury induced by oxidative damage and to claim its mechanisms at the molecular level.Methods:Peroxide hydrogen(H₂O₂) was used to induce injury of human umbilical vein endothelial cells(HUVEC) to duplicate the in-vitro free radical-injured model. The MTT assay was used to evaluate the cell survival rate in order to explore the protective effect of scutellarin against injury of vascular endothelial cells.On the above basis,the mRNA expression level of superoxide dismutase(SOD) and NADPH oxidase(NOX) was detected by the method of molucular hybridization in situ.The protein expression of SOD and NOX was assayed by the method of western blotting.Results:After incubated with 1 mmol/L H₂O₂ for 4 h,the viability of HUVEC significantly decreased(P＜0.05 vs normal control group).Scutellarin could concentration-dependently up-regulate the expression of SOD in HUVEC injuried by H₂O₂(P＜0.05 vs model group);scutellarin could concentration-dependently down-regulate the expression of NOX in HUVEC injuried by H₂O₂(P＜0.05 vs model group).Conclusion:Scutellarin showed obvious protective effects against vascular endothelial cells induced by oxidative stress and the probable mechanisms may be closely associated with its promoting the capability of antioxidases,down-regulating the expression of NOX protein and mRNA and up-regulating the expression of SOD protein and mRNA.