Research On Interstitial Cells Of Cajal And Substance P In Gastric Antrum Of Fatty Liver

Objective To observe the changes of interstitial cells of Cajal(ICC)and substance P(SP) in gastric antrum in fatty liver rat model,then to investigate the related mechanism of gastric motility changes in fatty liver.Method 1.Thirty SD rats were selected and randomly divided-into fatty liver group(n=20) and normal control group(n=10).2.Fatty liver model were duplicated by high-fat diet feeding.The hepatic tissue were sliced for pathological observasion.3.Using gastrogavage with radioactive ^99mTc to quantify liguid food emptying rate after three months.The tissue specimens were obtained from the rats of control group and the fatty liver model group.Immunohistochemical methods were used to investigate changes of the C-kit positive interstitial cells of Cajal and substance P in rat gastric antrum.Using quantitative image analysis software for statistical analysis.4.To observe the ultrastructure of ICC by electron microscope. Results Duplicating successful models of fatty liver.Compared with the control group,gastric transmission rate of fatty liver rats was significantly reduced(P＜0.05).C-kit positive interstitial cells of Cajal was reduced(P＜0.05),the expression of substance P decreased(P＜0.05).Electron microscopy exhibite that the junctions between interstitial ceils of Cajal and other cells were decreased and the structures were damaged.,and organelles decreased significantly,mitocnondria swelled,vacuoles formed.Conclusion The intestinal motility of rats with fatty liver was markedly delayed,which may be associated with the changes of interstitial cells of Cajal number and ultrastructure in gastric antrum;This also may be associated with the decreased expression of substance P.The topics laid a experimental basis for the study of the pathogenesis of delayed gastric motility in fatty liver,and even provide a new direction for clinical treatment.