Correlation And Expression Of C-myc And HBx Protein In HBV-associated Hepatocellular Carcinoma

Objective:To investigate the correlation and expression of C-myc protein and HBx protein in HBV-associated Hepatocellular carcinoma and the correlation of C-myc protein,HBx protein and serum HBeAg in the different developing stage of HBV-associated Hepatocellular carcinoma and to investigate the molecular mechanism of the occurrence and development of HBV-associated HCC.Methods:Expression of C-myc protein,HBx protein were detected in the tissues of nomal liver,chronic hepatitis,liver cirrhosis,paratumor cirrhosis and HBV-associated HCC(72 samples,totally)by sp immtmohistochemistry. Expression of HBV serum markers were detected by ELISA.All data were processed by SPSS 11.5 version in the computer.Results:C-myc protein was localized in nuclear.The expression of C-myc protein in paratumor cirrhosis was strongest in the five groups(p＜0.05).Next came HBV-associated HCC(p＜0.05).The correlation between C-myc protein expression and differentiation degree of HBV-associated HCC was checked with Spearman method and had postive relationship(r=0.583,p＜0.05).HBx protein was localized in nuclear.The expression of HBx protein in chronic hepatitis B(p＜0.05).Next came HBV-associated HCC(p＜0.05).The correlation between HBx protein expression and differentiation degree of HBV-associated HCC was checked with Spearman method and had no relationship(p＞0.05).The correlation between C-myc protein expression and HBx protein expression in HBV-associated HCC was checked with Spearman method and had postive relationship(r=0.738,p＜0.05).Conclusions:C-myc protein was closely related to the occurrence and development of HBV-associated HCC.C-myc protein overexpression may participate in the starting of HBV-associated HCC.The correlation between HBx protein expression and differentiation degree of HBV-associated HCC had no relationship. HBx protein can promote the espression of C-myc protein.