Interfere SDF-1 Signaling Pathway Delay To Reduce Degeneration Research With Hyaluronic Acid In Vitro

[Objective]Our experiment is to definite the role of the signaling pathway of SDF-1 in articular cartilage degeneration and to explore the feasibility that interfering SDF-1 signaling pathway delag the organization of deqeration of artcular cartilage and further to prove the HA's pharmalological mechanism in treatment for bone and patients.[Methods]1.Obtaining the intro-articular synovial fluid from the patieity of Osteoarthritis(Osteoarthritis,OA) in patients with intra-articular synovial fluid from the patients of OA,centrifugation to remove detached cells and residues,and use the filter after filter,Determination of synovial fluid within the SDF-1 concentration,-80℃refrigerator deep hypothermic preservation of the reserve.2.Obtaining the cartilage in size of 3×3×1mm as.The test group in tolas knee replacement surgery.Another result of traumatic amputation with normal knee cartilage surface of six case in the cut size of 3×3×1mm pieces of cartilage(Mankin score of 0 or 1) for the control group;get the fetus knee cartilage(induced by the cystic law the fetus was induced) size 3×3×1mm of tissue(Mankin score of 0 or 1) compared with the pilot training,the culture medium containing different concentrations of OA synovial fluid(containing SDF-I),OA synovial fluid (containing SDF-1) mixed with the HA culture medium,37℃CO2 incubator to observe training.3.The experiments of cartilage tissue in vitro 48 h and 96 h later,the cartilage tissue samplesand conditioned medium were tested by collected for histological observation(HE and red Pan-O(Safranin-O) staining),with Mankin score standards organizations Science score;measured in the culture medium of matrix metalloproteinase-3(Matrix metalloproteinases-3,MMP-3),-9,-13 of the content.The data was analgsed by spss12.0 to compare the score differences in each group have statistical significance.[Results]1.In the synoviat fluid of OA patients to high concenfialions the cartilage lycopene HE and red O staining results of observation,the number of cecls is little and cytoplasmic staining is uneven;The synorial fluid of OA patients with loue concentrations of through HE and red O,number of cell than the previous group,a more uniform cytoplasmic staining.OA synovial fluid(containing SDF-1) high concentrations of the group with the cartilage tissue culture fluid within MMP-3,9,13 factor content higher than OA synovial fluid(containing SDF-1) low concentration group.2.The experimental group with culture medium containing SDF-1 in OA synovial fluid mixed with the HA and the cartilage tissue lycopene HE red O results of observation,that is,the control group of experimental culture medium containing SDF-1 in synovial fluid of OA Group Results in comparison,the experimental group was significantly better than the control group with OA synovial fluid(containing SDF-1)and blank control group that is the low concentration group;blank control group,histological observation,the experiment is superior to the control group. Through histochemical observation of the results of the experimental group of typeⅡcollagen content of the experiment is superior to the control group and blank control group,blank control group,typeⅡcollagen content of the experiment is superior to the control group.Detection of culture medium experimental group MMP-3,9,13 to the content of the experiment was significantly lower than the control group and blank control group,blank control group,MMP-3,9,13 the content must be less than the control group.[Conclusion]1.SDF-1 signaling pathway can induce degeneration of knee cartilage.2.Hyaluronic acid can interfere with SDF-1 signaling pathway in order to delay the degeneration of articular cartilage.