The Effects On Proliferation And Differentiation To Chondrocytes Of SD Rat Bone Mesenchymal Stem Cells In Vitro Of Ginsenosides Rh1

Objective:To establish steady culture and multiplication procedures of bone mesenchymal stem cells(BMSCs) from SD rats,observing BMSCs growth and biological characteristics and the effects of ginsenosidesRh1 on their proliferation and differentiation to chondrocytes,exploring the feasibility of differentiation to chondrocytes culture by ginsenosidesRh1.Methods1.BMSCs from SD rats were isolated and purified by using different attachment method,then indentified according to morphology and induced-differentiation potentiality.2.MTT method was used to investigate the proliferation effects of different concentrations of ginsenosidesRh1 on BMSCs in SD rats,screening the optimal concentration of ginsenosidesRh1.3.The most effective concentration of ginsenosidesRh1 were used in our experiment,4 groups were divided:blank control,classic group(included TGF-β1,Dex,Vtic),ginsenosidesRh1 group,ginsenosides Rh1+classic group.The chondrocyte identification and induction rate were observed by collagen typeⅡimmunohistochemical staining,the content of glycosaminoglycam(GAG)were measured by DMB,evaluating the effect of induction and differentiation.Results:1.Primary cultured BMSCs were adhered to plastic surface within 48 hours and reached confluence within 10-15days.We can obtain the homogeneous P3 to do experiment by changing culture medium.To be conditioned induced,BMSCs were successfully differentiated into osteoblast,adipose,and chondrocyte.2.MTT indicated that the concentration of ginsenosidesRh1 between 10mg/L-50mg/L played a role in the proliferation of BMSCs,especially in the concentration of 25mg/L,which had statistical significance compared with blank control((p＜0.05). 3.After 14 days of BMSCs induction,the induction rate in classic group and ginsenosidesRh1+classic group were higher both than blank group and ginsenosides Rh1 group respectively,p＜0.05;4.After 14 days of BMSCs induction,the content of GAG in classic group and ginsenosidesRh1+classic group were higher both than blank group and ginsenosides Rh1 group respectively,p＜0.05.Conclusion:1.Using different attachment method could obtain purified BMSCs in SD rats.2.GinsenosidesRh1 could promote the proliferation of mouse BMSCs.3.BMSCs cultured with ginsenosidesRh1 could not be differentiated into chondrocyte.however,ginsenosidesRh1 can assistant and strengthen the differenttiation action of the classic groups.