Expression Of OX40 And OX40LmRNA On Peripheral Blood Mononuclear Cells Of Patients With Acute Coronary Syndrome

Objective To compare the expression of OX40/OX40L mRNA on Peripheral Blood Mononuclear Cells in patients with various clinical stages of atherosclerosis(stable angina and acute coronary syndrome) and to better understand the association of OX40/OX40L with the progression and deterioration of coronary artery disease.Method A total of 170 cases(include controls:30,stable angina:60,acute coronary syndrome:80) were investigated.Blood samples were obtained from all cases and the expression of OX40/OX40L mRNA on Peripheral Blood Mononuclear Cells were determined.All patients were identified with coronary angiography(CAG).All coronary stenoses with≥50% diameter reduction were assessed by angiographic coronary stenosis morphology.Result(1) The expression of OX40 mRNA on Peripheral Blood Mononuclear Cells in patients with ACS group(0.72±0.15) were significantly higher than SAP(0.29±0.09) and control(0.24±0.06) groups (P₁＜0.05,P₂＜0.01).But there was no different significance between SAP group and control group(P＞0.05);(2) The expression of OX40LmRNA on Peripheral Blood Mononuclear Cells in patients with ACSgroup(0.50±0.15) were significantly higher than SAP(0.25±0.10) and control(0.20±0.11) groups(P＜0.05).But there was no different significance between SA group and control group(P＞0.05);(3)The score of angiography morphology of coronary lesions in ACS group was significantly higher than SAPgroup(3.89±0.92 VS 1.32±0.61,P＜0.05). (4) The levels of OX40L mRNA were correlated with the coronary severity scores(r=0.59,p＜0.05);Correlation analysis suggested that the expression of OX40mRNA were positively correlated with the coronary severity scores(r=0.76 p＜0.01).Conclusion There is a small number of basic OX40 mRNA and OX40LmRNA expression on peripheral blood Mononuclear Cells in human.The increased levels of OX40 and OX40L mRNA in ACS may enhanced the inflammation,aggravated the development of atherosclerosis and mediated the rupture of instable plaque.It can be served as a valuable marker for evaluating the degree of coronary artery pathology and predicting clinical risk in ACS.