Experimental Study On Nerve Allografts Stored In Green Tea Polyphenol Solution

Objective To explore the outcome of repairing nerve defects with nerve allografts stored in green tea polyphenol solution。Methods 84 healthy Wista male rats were used as the experimental.60 male rats were randomly divided into 5 groups of nerve grafting,with 12 rats in each group.A 1.0 cm sciatic nerve segment,5mm away from infrapiriform foramen,was removed and repaired by 4 kinds of nerve allografts.Group A: autografts;Group B:allografts;Group C:cryopreserved nerve allografts; Group D:nerve allografts stored in green tea polyphenol solution;Group E: radiated nerve allografts.At the point of 6 weeks and 12weeks,a series of examinations were performed,including:the gross appearance,electro -physiological test,histological observation,electron microscopic study,quantitative analysis with image analysis system.Results All the examinations of group A and D were better than group B,C and E(P＜0.05),but there were no statiscally significant differences between group A and D.And there were no statiscally significant differences between group C and E.Conclusion1.The nerve allograft stored in green tea polyphenol solution is a good substitute for nerve graft.2.The nerve allograft stored in green tea polyphenol solution is a better nerve graft than the cryopreserved one.3.The nerve allograft stored in green tea polyphenol solution is a better nerve graft than the radiated one.4.The results of cryo-preserved and radiated peripheral allogeneic nerve grafting are alike in 12 weeks.