The Experimental Study Of Radiotherapy On Echinococcosis

Objective:To demonstrate the possibility and mechanism of radiotherapy by investigating the effects of different doses of 6MV X-ray radiotherapy on Echinococcus granulosus in rat.Methods:After being irradiated by 10,20,30 and 40Gy of 6 MV X—ray,these examinations were carried out such as the ultrastructure of the Echinococcus granulosus cysts in rat with electron-microscope,the total protein amount in hydatid cyst fluid(HCF) and Ca²⁺ ion in HCF.The potassium-pyroantimonate(PPA) cytochemical method was used to demonstrate whether blocked calcium channels would be one reason of radiotherapy on Echinococcus granulosus cysts in rat.Results:The cyst wall tissue and cells appeared abnormal changes and damages microscopically in the treated groups to a different extent.The total protein amount in hydatid cyst fluid(HCF) increased,while Ca²⁺ in HCF reduced obviously in the treated groups,especially in high dose groups.With PPA,some electron-dense precipitates were presented on the mitochondria and endocytoplasmic reticulum in the treated groups.Conclusion:The structure of the cysts of Echinococcus granulosus in rat can be damaged by radiotherapy which showed a quantity-efficiency relation to some extent.