Variants In Insulin-degrading Enzyme Gene And Metabolic Syndrome

[Objective]To identify specific genetic risks for metabolic syndrome(MetS) associated with insulin-degrading enzymem(IDE) gene.[Methods]A case-control association study was performed in 563 Chinese elders in Shanghai China.Cases were those with MetS(n=241) and controls were those without any component of MetS(n=219).103 subjects diagnosed type 2 diabetes mellitus(T2DM) were recruited for secondary comparison.Five unrelated genetic markers(single nucleotide polymorphism,SNP) at the IDE gene were used for association analyses.Weight,height,systolic and diastolic blood pressure were measured.Venous blood was collected after a minimum 10 hour fast;fasting plasma glucose,postprandial 2h plasma glucose, plasma total cholesterol,serum triglyceride,high density lipoprotein cholesterol(HDL-C) and low density lipoprotein(LDL) were tested using an auto biochemistry instrument.Plasma fasting insulin level was teted by radio-immunoassay.As a marker of insulin resistance,homeostasis model assessment(HOMA)-insulin resistance(IR) was used and the calculation formula was as follows:HOMA-IR=[fasting insulin(μIU/mL)×fasting glycemia (mmol/L)]/22.5.[Results]The single locus association analysis revealed the A/T allele of rs11187033 was associated with MetS(OR=0.698,95%CI:0.526-0.928,P=0.013). A/T allele carriers were easily to develop MetS(B=2.329,0R=4.888,P＜0.001) and had higher BMI,HOMA-IR and TG values than A/A and T/T carriers at rs11187033.MetS patients had more haplotype G-T-T than controls and G-T-T carriers were more easily to develop MetS(OR=1.733,P=0.008).Less G-T-A carriers developed MetS(OR=0.747,P=0.036).None of the other 4 SNPs were significantly associated with MetS.There was no statistical difference in genotype distribution between MetS patients with and without T2DM/IGT. [Conclusions]The rs11187033 at IDE gene might contribute to MetS susceptibility in Chinese elders.A/T allele at rs11187033 might work in the way of affluence the process of insulin resistance and obesity.