The Expression Of SDF-1 And VEGF In Oxygen Induced Retinopathy Mice Model And The Intervention Effect Of AMD3100

Abstract:Objective:In order to investigate the expression of vascular endothelial growth factor(VEGF) and stromal cell-derived factor-1(SDF-1) in the retinas of the mice,having oxygen-induced retinopathy.Also to evaluate the inhibition of an antagonist to the neovascularization in the OIR mice.Method:We included 58 C57BL/6 seven-day-old mice.29 of them were composed as the control group,while the others were formed as the test group(OIR mice model).We divided all the mice into 6 groups:control group(n=17):6 17-day-old(P17) mice were sacrificed to have retinas ADPase stained and to be made into paraffin sections with HE staining. 6 19-day-old(P19)mice were handled as P17.We used another 5 P19 mice retinas to quantify the mRNA expression of VEGF and SDF-1 by Realtime RCR. The test group(n=17) were processed like the control group.According to the dosage of AMD3100,other mice(n=24) were divided into low dose group,high dose group,low dose control group,and high dose control group (each group n=6,contralateral eyes were intravitreal injected BSS as self-control group).Each group(P19) were sacrificed to make ADPase staining,paraffin sections,and immunohistochemical staining(anti-VEGF and anti-SDF-1).We measured the average positive staining area percentage(APSAP) as the outcomes and processed with t test.Result:Realtime PCR showed expression of both VEGF mRNA and SDF-1 mRNA in retinas of both the control group and test group.Moreover the test group's expression is significantly higher(t=2.488,P=0.038;t= 2.864,P=0.021).The ADPase staining sections and paraffin sections of the intervented group are more closer to normal than those of the test group.Immumohistochemical staining sections showed that VEGF and SDF-1 expressed in neuroepithelial in each group.APSAP evaluated,the intervented group showed significantly lower than the self-control group.Conclude:Realtime PCR indicated an increased expression of VEGF and SDF-1 in the OIR model retinas.The AMD3100 intravitreal injected model retinas had less neovascularizaion,and the expression of VEGF and SDF-1 protein decreased.These results hinted that AMD3100 antagonised the receptor of SDF-1 to weaken the effect of the combination of SDF-1 and CXCR4 so as to reduce VEGF protein and inhibit neovascularization.