The Sequential Changes Of Hypoxia-inducible Factor 1 Alpha In Secondary Spinal Cord Injury And Its Significance

ObjectiveTo investigate the sequential changes of hypoxia-inducibal factor 1α(HIF-1α) in experimental secondary spinal cord injury in rats and its potential effects on SSCI.Methods66 SD rats were divided into 11 groups randomly(normal control group, pseudoinjury group,and SCI groups at 1h,3h,6h,12h,1d,3d,5d,7d,14d after injury respectively).The static compression model of SCI was built in the injury groups,and only laminectomy was applied in the pseudoinjury group.The spinal cord of T₁₀ was injured in the SCI groups.Expression of HIF-1αwere measured with HE and immunohistochemical staining.The positive cells were counted and analysed by SPSSV11 software.ResultsHIF-1αwas poorly expressed in normal spinal cord cells.The expression increased 3h after injury,and reached peak at 1～3d.One week later,it declined significantly.The positive expression was found in spinal cord neuron,ganglion cells and vascular epithelial cells in the stroma.ConclusionThe expression of HIF-1αincreases after SCI,that is controlled by the pressure of oxygen.And it is related to the time after SCI.HIF-1αcan protect the anoxic tissue and cells..