| Objective: Explore influence ofβ-interferon on inflammatory response and recovery of neurological function after acute spinal cord injury in rats to provide experimental basis for the treatment ofβ-interferon in SCI.Methods: 1.SD rats with 64 were randomly divided into 4 groups, including A group (SCI group); B group (MP group); C group (IFN-β)and D group (sham operation group), each groups 16 rats.2.The segments T9-10 of spinal cord were impact with home-made device of Allen to set up models of spinal cord injury. Each group with different treatment after injury: After SCI, A group will not do anything; B group immediately injects 30mg/kg MPSS via the tail vein for treatment; C Group immediately injects IFN-β(1×107 IU )and 4h after injection injury IFN-β(0.5×107 IU) via the tail vein to interven; D group which is the sham operation group will not be given any treatment.3.24h after surgery, the materials of T9-10 will be drawn from the center of injury, cut 1.2cm long of cord tissue including the head and end.IL-1β, IL-6, TNF-αgene expression in each group of spinal cord injury are assayed with RT-PCR;MCP-1 positive cells and CD68 positive cells (macrophages) in the spinal cord regional distribution of injury are assayed with immunohistochemistry.4.The methods of BBB score, climb and swim grid experiments in rat behavioral experiments to assess changes in the recovery of neurological function of spinal cord.5.SPSS13.0 software is used for statistical analysis of experimental data.Result: 1.RT-PCR results showed that: IL-1β, IL-6, TNF-αin spinal cord with no injury expressed weakly , but expressed significantly increased after SCI.Each gene expression decreased after methylprednisolone treatment intervention, andβ- interferon-treated group decreased more significantly.2.Immunohistochemistry results revealed: There were only few CD68 positive cells and MCP-1 positive cells which were distributed in spinal cord with no injury. CD68-positive cells and MCP-1 positive cells were markedly increased after SCI and methylprednisolone can reduce the CD68 positive cells and MCP-1 positive cells in the injured spinal cord tissue butβ-interferon reduced those cells more significantly.3. Behavioral score shows:There is significant correlation between inflammatory response after spinal cord injury and the degree of restore of neurological function. The dual-hindlimb immediately paralysis after spinal cord injury in rats, but with the time passage there were varying degrees of improvement.The function of hindlimb in rats improved the worst in A group and B,C groups were better than the A group.Conclusion:β-interferon reduce IL-1β, IL-6, TNF-αmRNA expression and CD68, MCP-1 positive cells assmeble in tissue of spinal cord injury, so reduce the inflammatory reponse in spinal cord injury. Meantime improve neurological function in rats after spinal cord injury. The drug ofβ-interferon is simple, safe, effective and feasible for the treatment of acute spinal cord injury in rats. |
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