Methodology Research On Effective Extraction And Purification Of Shikimic Acid From Picea Meyeri

There was shikimic acid in Picea meyeri by determining.Take a series of technique for extraction and purification of shikimic acid.Thus it can apply generally in medicine.Shikmic acid was a important intermediate product to compound Oseltamivir Phosphate which was resisting bird flu.Besides it had a series of pharmaco-function,such as anti-inflammatory, analgecize,pntibiosis,anticancer,restrain tarombokinesis,restrain insufficient blood to the brain.The dissertation made research by the numbers on effective extraction and purification of shikimic acid from Picea meyeri.The primary coverage was as follows.Set up HPLC method which was for determining the content of shikimic acid. Chromatographic column:AichromBond-AQ C18(4.6mm×250mm,5μm),mobile phase: 0.01%H₂SO₄-H₂O,velocity of flow:1.0ml/min,column temperature:25℃,detection wave length:213nm,sampling dose:10μl.It had made sure the optimization of the extraction technology of shikimic acid from Picea meyeri by response surface methodology.Using refining method to extract shikimic acid from Picea meyeri.By comparing with four frequently-used extraction methods,choose refining method as the extraction method.To optimize the one factor which is in the extraction process by the method of Box-Benhnken.Take the extraction rate and purity as index.Finally get the condition of optimum methodology was as follows:extraction temperature was 40℃, extraction time was 3min and the ratio of material to solvent is 1:10.The extraction rate is 0.84%and the purity was 6.15%.Have reseatch on the methodology parameters of sepatation shikimic acid from extract water of Picea meyeri by ion exchange resin.Eight different kinds of ion exchange resins named D261,D296,D301-R,D301-G,D290,201*7(717),D201 and D280 were studied on the adsorbance,adsorption rate and desorption rate with shikmic acid,the better resin is D290.The condition of optimum methodolgy which was to separate shikimic acid with D290 as follows. Adsorption condition:pH 6,temperature 25℃,the flowing rate was 3ml/min;desorption condition:eluent is 2.5%NaOH aqueous solution,the flowing rate was 3ml/min.With the condition of optimum separation and purification,the adsorption rate was 83.35%,desorption rate was 93.65%,the yield of shikimic acid was 85.06%,the purity was increased from 6.15% to 21.76%with 3.6 times as high as the crude extracts.Research on the methodology of purification shikimic acid by column chromatography of silica qel.The concrete methodology condition was as follows:the filler of column chromatography was 100-200 silica qel;loading amount was 0.4g/10g silica qel;eluting solvent is acetic ether-aether petrolei-formic acid 7:2:1;the flowing rate was 3ml/min;getting the corresponding eluting solvent together.8g sample which was separated from extraction shikimic acid by column chromatography had 72.14%purity 1.5g and 78.21%yield.