Antibacterial Structure And Activity Investigation Of Human Semenogelin â…  Derived Peptides

Objective:To study the relationship between changes of primary,secondary structure and antibacterial activity of human Semenogelin I derived recombinant peptides.Methods:On the basis of functional peptide SgI-29 which have biological activity in the past experiments,four small recombinant peptides were designed and synthesized by replacement and deletion the amino according to the feature of antimicrobial peptide and known structure activity relationship.Physico-chemical characteristics and structure prediction of SgI-29 and its derived peptides were analyzed by software packages and helix-wheel plot.The antibacterial activities of SgI-29 and its derived peptides against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 were determined.The structure-function relationship of SgI-29 and its derivatives was analyzed.Results:SgI-22 has the strongest antibacterial activities against the tested bacteria among the synthetic peptides and might be used as a good model for the structure optimization.Stable ahelix, bigger hydrophobic moment,concentrated net charge distribution and suitable hydrophobicity are important elements of antibacterial activity.