| BACKGROUND:The studies of cardiac makers had been made developed by leaps and bounds in the pasted several years,and new ones have been found in groups such as ischemic-modified albumin(IMA), heart fatty acid-binding protein(H-FABP),glycogen posphorylase isoenzyme BB(GPBB) et al.Now the cardiac makers are used for the diagnosises of the necrotic injure of myocardium,but are seldom applied for the injure caused by the relative ischemia of caused myocardium due to the rapid increase of the demand of blood volume in the heart of healthy body.The markers which reflect irreversible myocardial damage are chosen too much for the existing experiments,so the diagnosis rate of reversible injure are lower and the injury of myocardium can not be discovered in the early stage.OBJECTIVE:The aims of studies are classified as below:To analyze the relation between exercise and myocardial damage,to determine,compare and assess the early stage makers of ischemic and hypoxia myocardial damage,To investigate the role of the factor of energy metabolism in the myocardial damage,to seek ways to reduce the injury of myocardium and to weigh the protective action of L-carnitine during the exercise.METHODS:Sixty young health men lacking of long-term endurance training are divided into two groups:control group and medicine group.The men in control group make cross-country running of long distance on empty stomachs,with their blood drawn and serum dissociated at three different times for test:before exercise,instantly after exercise and 4 hours after exercise.The medicine group taking L-carnitine in 1.0 gram,3times per day before the exercise,and then do the same procedure.The serum are tested for the concentration of ischemic myocardium makers including ischemic modified albumin(IMA),heart-fatty acid binding protein(H-FABP),glycogen phosphorylase isoenzyme BB(GPBB),myoglobin(MB),creatine kinase-MB and tropinin-T(CTnT).At the same time the following biochemical indexes are detected:blood glucose(GLU),total cholesterol (TC),triglyceride(TG)and free fatty acid(FFA).Compare the value of the indexes between pre-exercise and post-exercise and analyze the effect of L-carnitine on the myocardium in the exercise.RESULTS:The value of GLU in serum arose significantly in both medicine and control group(P<0.05),and returned to normal level in 4 hours after exercise(P>0.05);the value the medicine group was significant higher than the one of the control group.The concentration of FFA decreased significantly on instantly after the exercise and 4 hours post-exercise in control group(P<0.01),but didn't in the medicine group (P>0.05).The values of CK-MB before and after the exercise in two groups neither had remarkable change,but the value of medicine was lower than the control group.Before and after exercise,there were not significant changes about CTnT in both groups.The IMA values in control group did not have obviously changes among before the exercise, the instantly after exercise and 4 hours after exercise(P>0.05).Compare to control group,the IMA value of the medicine group in the three times is lower obviously,the differences are significant(P<0.01).The value of GPBB in control group arose remarkably on instantly after exercise and 4 hours after exercise related to pre-exercise.So did the value in medicine group(P<0.01).Compare between two groups,the value in the medicine group in the three times is lower(P<0.05).In control group,the GPBB value arose obviously on instantly and 4 hours after sporting from before sporting.While only arose on 4 hours after exercise in medicine groups. When compared between two groups,relative the value of GPBB reduced in the medicine group(P<0.05).In control group,on the 4 hours after exercise the value of H-FABP arose(P<0.01).In medicine group the rise extent is higher(P<0.01).In both groups,the values of Mb on instantly after exercise and 4hours arise a little,the changes were not significant (p>0.05).CONCLUSION:The cross-country run of 5000 meters has different effects on the substance which providing energy for myocardium:it induced the blood glucose to arise,make the FFA in the serum become lower.There are not obviously action on the myocardial makers often used,but the influence on the new ones including, heart-fatty acid binding protein(H-FABP) is significant when compare the results pre-exercise,instantly after exercise and 4 hours after exercise,in which the change range of glycogen phosphorylase isoenzyme BB(GPBB) is more wider.Taking L-carnitine can prevent the myocardial makers from arising due to exercise. |
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