Expression Of TIP30/CC3 Gene In Transitional Cell Carcinoma Of The Bladder And Its Significance

Objective: Bladder cancer is the most common malignant tumor in genito-urinary system. Epithelial tumors account for more than 95%, and the majority are transitional cell papilloma. In accordance with the depth of invasion, bladder cancer is divided into non-muscle invasive bladder cancer and muscle invasive bladder cancer. Although non-muscle invasive bladder cancer have a lower degree of malignancy, the recurrence rate after operation is high. And there is around 16%-25% of recurrent tumors which degree of malignancy increase. There are about 10 percent of that which develop into invasive cancer or metastatic. Muscle invasive bladder cancer are often poorly differentiated tumors with high-level and the basic treatment is the radical resection of the bladder. After the operation, the patients have a poor qulity of life and bad prognosis. The reports that 5-year survival rate are more than 50% are rare. Therefore, in-depth study of the recurrence of bladder cancer and its transfer mechanisms, looking for more effective diagnosis and treatment are important issues which are urgently needed to resolve .TIP30,also called CC3,is a tumor supperessor. It was originally identified by a differential display analysis of messenger RNA from the highly metastatic human variant small cell lung cancer versus less metastatic classic small cell lung cancer lines. Its transcription product exist widely in the heart, brain, lung, kidney, skeletal muscle and pancreas and other normal tissues and some tumor cell lines such as colon cancer, neuroepithelial tumors, melanoma and Burkitt lymphoma cell lines. And the expression in a variety of tumor tissues significantly reduced, such as melanoma, malignant glioma, breast,colon and hepatocellular carcinoma. TIP30/CC3 imbalance have direct relationship with the tumor. This research detect the expression of TIP30/CC3 in normal bladder tissue and TCCB.This may represent the possible effect in bladder cancer.Methods: SP immunohistochemical method was used in detecting TIP30/CC3 in 80 TCCB and 12 normal bladder tissues. (1)The expression of TIP30/CC3 in TCCB tissue of various clinical stage,pathological grade,sex and age were studied. (2)The expression of TIP30/CC3 in primary tumors and recurrent tumors were studied.Result: 1.Immunohistochemical detection of TIP30/CC3 protein in bladder cancer tissue and normal bladder tissues.In bladder cancer with the positive expression of TIP30/CC3 cytoplasm showed yellow granules or clumps. TIP30/CC3 protein can be detected in normal bladder tissues and cancer tissues. In 12 cases of normal bladder tissues the positive expression rate of TIP30/CC3 was 83.3%(10/ 12) , in 80 cases of bladder transitional cell carcinoma tissues the positive expression rate was 42.5%(34/80),the difference between the latter and the former was significant(P < 0.01).2. The relationship between the TIP30/CC3 protein expression and the clinical indicators.Comparing with the positive expression rate of TIP30/CC3 gene of the non-muscle invasive bladder cancer(42.5%), that of the invasive bladder cancer(22.2%)lowered.The difference was marked. Both the expression of grade 2 bladder cance(r45.5%)and grade 1 bladder cance(r57.9%)were higher than that of grade 3 bladder cance(r17.6%). The difference was marked. The expression of recurrent cancer(21.7%)was lower than that of primary cancer, and the difference was marked. Statistics indicate that the expression of TIP30/CC3 is irrelevant to the sex and age.Conclusion:1. TIP30/CC3 gene show low expression or express deficiency in TCCB. 2.TIP30/CC3 gene positive expression rate in TCCB lower accompanying with the increase of clinical stage, histological grade. 3.TIP30/CC3 gene may play important roles in suppressing the tumor gensis and development in TCCB. They are possible candidate suppressor gene in TCCB.4.TIP30/CC3 gene is related with the recurrence of TCCB. It may become one of the indexes of detecting tumor recurrence.