Cellular And Humoral Immune Responses Induced By HIV-1clade C/B-MVA Vaccine

People's life and health was threatened seriously with the high spread pace of HIV virus.Affordable therapeutic strategies that induce sustained control of human immunodeficiency virus type 1(HIV-1) replication and prevent HIV infection are urgently needed Vaccinia virus vector was a popular HIV vaccine recently which inducing better cellular immune responses.And CD8~+and CD4~+T cells are crucial to HIV-1 control.The object of the research is evaluating the immunogenicity of a recombinant modified vaccinia virus Ankara-vectored vaccine expressing the consensus HIV-1 clade C/B'.Mice were inoculated with 3-dose HIV vaccine by intramuscular injection.Blood sample were collected every second week,and then the antibodies against HIV were detected.At week 6,mice were killed and cellular immune responses were examined by ELISPOT.Result has shown the number of spot forming cells in the 10~7 pfu/mL -dose group was more than those of 10~5 pfu/mL -dose and 10~6 pfu/mL -dose groups significantly.HIV specific antibodies emerged at week 2 and elevated rapidly at week 4 and week 6.The level of specific IgG in the 10~7 pfu/mL -dose group was more than those of 10~5 pfu/mL -dose and 10~6 pfu/mL -dose groups significantly.Conclusion The ADMVA induces both humoral immunoresponse and cellular immune responses.