Investigation Of Hematotoxicity Of Benzene And Its Metabolites

PurposeThrough on-site investigation and in vitro study of benzene and its metabolites on the blood system and the role of part of the mechanism of injury, and explore the benzene exposure levels in workers exposed to blood and peripheral blood system cells, some of the indicators and related biochemical indicators in order to enrich the blood of benzene The toxic mechanism of the system.Methods1,First part Select a shoe factory next 57 benzene workers and 26 non-workers access to the study, using high performance liquid chromatography (HPLC) determination study class at the end of SH-urinary uric acid content (creatinine correction), and The number of peripheral blood cells, lymphocytes structure of the blood in the cytochrome oxidase and glutathione S-transferase activity changes observed analysis of the relationship between the two.2,Second part Through exposure in vitro experiments, using micronucleus, chromosome G-banding and comet detection methods to observe the different concentrations of benzene metabolite of hydroquinone, 1, 2, 4 - phloroglucinol separate and combined effects of human peripheral blood lymphocytes Chromosome structure and DNA damage.Results1,First partCompared with the control group, the exposed group's blood indicators have a declining trend, and serum CYP450 activity has a increasing trend, but the statistical analysis shows that the indicators'difference are not significant. Each group of the average serum GST activity: high-exposure group is 20.73μ/ml, low-exposure group is 22.62U/ml, the control group is 17.78μ/ml, among the three groups have a significant difference (p=0.003), two-two comparison show that high-exposure and control group,low-exposure and control group are significantly different (p=0.003), while the two exposure group reveal no statistically significant difference (p=0.975). Micronucleus rate of lymphocyte in the peripheral blood of high-and low-exposure and control group are respectively 9.00‰,4.00‰,2.00‰, the analysis found that among the three groups have statistical significance (p<0.01), moreover the micronucleus rate and the concentration of urine S-PMA have a clear dose-response relationship (r=0.678, p<0.001). Between the two exposure group, the concentration of air benzene and urine S-PMA are not significantly different, but they have a remarkable dose-response relationship (r=0.95, p<0.001), the length of service and micronucleus rate also exist good dose-response relationship (r=0.515, p=0.002).2,Second partHydroquinone, 1,2,4-Phloroglucinol ecposure and a separate joint exposure, micro-lymphocyte cells (MN) rate of lymphocyte chromosome aberrations (CA), lymphocyte DNA damage and there is an obvious dose of Dose-response relationship, and the two mebabolites of exposure and exposure alone, compared to a significant increase of these two metabolites that exists between the synergies.Conclusion1,In the benzene-exposure operating circumstances, the concentration of air benzene and urine S-PMA,urine S-PMA concentration and micronucleus rate,the length of service and micronucleus rate, all have the good dose-response relationship; the changes of serum GST activity is a certain significance to early diagnosis of benzene poisoning.2,It suggests that BT and HQ could induce chromosome damage on human peripheral lymphocyte and have synergetic effect.Comet assay is a test DNA damage in peripheral blood lymphocytes sensitive indicators.