Isolation And Physiology Activity Investigation Of Collagen From Cyanea Nozakii

Cyanea nozakii is a kind of harmful jellyfish for marine ecosystem which bloom out in the Bohai Sea and Northern Yellow Sea of China. In fact, the jellyfish bloom and Harmful Algal Bloom are top two ecological disaster of Chinese marine ecosystem, threatening the fishery industry. In order to eliminate threaten of jellyfish bloom on the marine fishery industry of our country. It is desiderated to undertake the basic research about how to use it as a kind of new marine resource. From the environmental economic point of view, it is the best solution try to find the way to utilize it. About how to use it as a kind of resource, however, we know nothing. Nothing has been reported about the basic knowledge and information about basic components, as well as their function up to now.Collagen is a major component of extracellular matrix (ECM), and has a unique three-dimensional structure with a unique amino acid composition. Owing to wonderful properties of its low-antigenicity, easy-biodegradability and excellent biocompatibility, collagen is used more and more widely in areas of biological material development, food industry, cosmetic manufacture, and medical production as well.Recently, there have been many interests in establishing efficient techniques to isolate collagen from skin of fish, and skin, cartilage and soon from mammals, and to apply them in development of various kinds of biological materials. However, there has been no report about the collagen from the Cyanea nozakii, as well as its biological functions on adjuvant arthritis treatment.For the first time, the collagen from Cyanea nozakii has been investigated comprehensively. The isolation and purification methods have been established, and the potential physiologic activity of the collagen from Cyanea nozakii on the prevention and treating of adjuvant arthritis has also been explored. The data and results collected and recorded on this thesis can be used as the basic information for the further research and development of Cyanea nozakii, as a kind of new marine conllagen resources.The collagen from Cyanea nozakii has been extracted by enzymatic method. The collagen sample obtained was examined by PAGE and SDS-PAGE, which indicate that collagen from Cyanea nozakii may have the chain composition of a heterotrimer and the subunits weighed from 66.2 KDa to 116 KDa. Respectively, the percentage of hydroxyl-proline in the collagen after purification was 10.54%, the concentration of the protein was 98.0%, and the concentration of the polysaccharide was no detection.The acute toxicity experiment showed that injecting big dose, mice didn't die and hadn't obvious toxicity reaction. In a word, the collagen from Cyanea nozakii has no toxicity, which security dose was 15000 mg/kg.In this study, therapeutic effect of Cyanea nozakii collagen by oral administration on adjuvant arthritis in rats was initiatory explored. Cyanea nozakii collagen at different concentration could ameliorate the adjuvant induced arthritis, suppress the concentrations of NO and MDA in the blood serum and increase the activity of SOD in the blood serum. From these indexes can presume that, Cyanea nozakii collagen is probably ameliorating the adjuvant induced arthritis.