Expression And Significance Of BRCA1 And Ki-67 In Primary Epithelial Ovarian Cancer

Objective:To investigate the expression of BRCA1 and Ki-67 in epithelial ovarian tumor and the clinical significance in pathogenesis,progression and prognosis of primary epithelial ovarian cancer.Methods:Tissue microarray technology and immunohistochemical method was used to detect the expression of BRCA1 and Ki-67 in 62 cases of primary epithelial ovarian cancer,14 cases of borderline epithelial ovarian tumor and 30 cases of benign epithelial ovarian tumor.Results:â‘ The abnormal expression rates of BRCA1 in epithelial ovarian cancer (80.6%) were significantly higher than that in borderline tumor(14.3%) and benign tumor(0%) of ovarian.The positive expression rates of BRCA1 in epithelial ovarian cancer(59.7%) were significantly lower than that in borderline tumor(92.9%) and benign tumor(100%) of ovarian.â‘¡The present data demonstrated that the expression of BRCA1 was not significantly correlated with surgical-pathologic staging and histological types of ovarian cancer(P＞0.05),but correlated with pathologic grade of ovarian cancer (P＜0.05).The expression of BRCA1 in G₃ was31.6%,which was remarkably reduced than in G₁,G₂(78.6%and 69.0%).â‘¢Immunohitochemical analysis also showed that Ki-67 expressed increased in cancer(72.6%) than in borderline tumors(21.4%) or benign tumors(0%) of ovarian(P＜0.05).Especially,the strong positive expression of Ki-67 was striking increased in ovarian cancer(53.2%) than in borderline ovarian tumor and benign ovarian tumor(0%)(P＜0.01).â‘£A strong association has been observed between the expression of Ki-67 and surgical-pathologic staging,pathologic grade of ovarian cancer(P＜0.05),but not with hisological types(P＞0.05).The expression of Ki-67 was decreased in stageⅠ～Ⅱ(52.2%) than inⅢ～Ⅳ(84.6%).its levels were increased in grade G_2-3(75.9%and 94.7%) thanin G₁(35.7%).⑤.Expression of BRCA1 was negatively correlated with expression of Ki-67. Conclusion:â‘ In benign ovarian tumor,expression of BRCA1 was stained in cell nucleus.However,in primary epithelial ovarian cancer,expression of BRCA1 was abnormal,such as decrease or absent in nucleus and mislocated in cytoplasm.The results suggest that anormaly of BRCA1 protein expression may be an important event in pathogenesis of the epithelial ovarian cancer.â‘¡The expression of BRCA1 protein was correlated with pathologic grade of ovarian cancer,which indicated the level of BRCA1 protein expression may be an indicatrix to judge the malignant degree of ovarian cancer.â‘¢Ki-67 expressed increased in cancer than in borderline tumors and benign tumors of ovarian,which demonstrated that the expression of Ki-67 can reflect reproductive activity of malignant cell and may be an ideal indicatrix to discriminate the property of ovarian tumor.â‘£A strong association has been observed between the expression of Ki-67 and surgical-pathologic staging,pathologic grade of ovarian cancer,which indicates cell hyperproliferation is an important mechanism during the evolution of the ovarian cancer. The expression of Ki-67 may be an indicatrix to judge the malignant extent of ovarian cancer.⑤There is a significantly inverted relationship between the expression of BRCA1 protein and that of Ki-67 protein.The conjoined detection of BRCA1 and Ki-67 protein may be helpful to estimate malignant extent,judge prognosis and guide therapy of ovarian cancer.