Experimental Investigation Of The Reversal Effect Of SC On Human Multidrug Resistance Breast Cancer Cell Line

Objective:To explore the reversal effect of stellera chamaejasme L.crude extract(SC) on human multidrug resistance breast cancer cell line.Methods:The inhibition rate of different concentration of SC to MCF-7/ADM cells were evaluated with MTF assay.The inhibition rate of adriamycin(ADM) to MCF-7/S,MCF-7/ADM and MCF-7/ADM plus SC were evaluated with MTT assay. The IC₅₀ of different agents was counted.The MCF-7/ADM cells intracellular concentration of ADM and the expression of P-gp with and without SC was detected by flow cytometer(FCM).Results:SC at the concentration of 0.25 mg/ml had no significant cytoxicity to MCF-7/ADM cells.When 0.25 mg/ml SC was added along with ADM to MCF-7/ADM culture in vitro,the medium inhibited concentration(IC₅₀)was evidently reduced and ADM resistance was partly overcome.The reversal fold was 2.53.0.25 mg/ml SC could significantly increase the intracellular accumulation of ADM and decrease the expression of P-gp in MCF-7/ADM cells(P＜0.05).Conclusion:SC had reversal effect on the multidrug resistance in MCF-7/ADM cells.The reversal mechanism might be related to the down-regulation of the expression of P-gp.