Effect Of Tan â…¡A On The Endothelial Cell Injured By The Serum Of Patients Of Blood Stasis Syndrome Associated With Hypertension Disease

Object:To study the effect of TanⅡA on the vascular endothelial cell(VEC) injury induced by the serum of the patients of blood stasis syndrome(BSS) associated with hypertension disease,and discuss the mechanism of this effect further.Methods:The human umbilicus vein endothelial cells(ECV-304) was injured by the 15% serum of the patients of BSS associated with hypertension disease,then treated with different concentrations of TanⅡA(10μg/ml,20μg/ml,40μg/ml) for 24 hours.And the activity of the VEC was determined by MTT assay.The content of Malondialdehyde(MDA) in cell medium was measured by thlobarbiturie acid reaction and the activity of superoxide dismutase(SOD) measured by xanthosine oxidase method.The DNA damage of the VEC was determined by Single cell gel electrophoresis(SCGE).The expression of the mRNA of thrombomodulin(TM) and von willebrand factor(vWF) was determined by the reverse transcriptase-Polymerase chain reaction(RT-PCR).Results:Compare with the model group,the 20μg/ml and 40μg/ml TanⅡA can increase the activity of the VEC and decrease the MDA content in cell medium(P＜0.05),the 40μg/ml TanⅡA can increase the SOD activity in cell medium significantly(P＜0.01),all the three doses TanⅡA can reduce the cell DNA damage and decrease the expression of the mRNA of TM significantly(P＜0.01),and the 20μg/ml and 40μg/ml TanⅡA decreased the expression of the mRNA of vWF(P＜0.05).Conclusion:TanⅡA has a protective effect on the VEC injured by the serum of the patients of BSS associated with hypertension disease,and its possible mechanisms are antioxidant and reduce the damage of the cell DNA.