A Preliminary Study On The Role Of Endothelial Cells And Tissue Factor, And The Immunological Regulation Effect Of Sappan Wood's Water Extractives In Graft-versus-host Disease

PartⅠA Preliminary Study on The Role of Endothelial Cells and Tissue Factor in Graft-versus-host Disease【Objective】Detected the expression of tissue factor in human endothelial cell and the density of IL-2 in the cultured supernatant after mixed cultrued human endothelial cells with allogeneic lymphocyte in vitro.Through the detection we could get a preliminary outcome about the significance of tissue factor in GVHD.【Methods】Human endothelial cells and allogeneic lymphocyte mixed cultrued in vitro for 4-6 hours.Then we detected the ratio of tissue factor , the expression of tissue factor mRNA in the endothelial cells,and the concentration of IL-2 in the supernatant.Then we tested the expression of TF mRNA and the concentration of IL-2 with different concentration of CsA.These were detected by Flow Cytometry(FCM) , RT-PCR and ELISA.【Results】After human endothelial cells and allogeneic lymphocyte mixed cultrued in vitro for 4-6 hours compare with the negative control ,the level of tissue factor and the concentration of IL-2 increased (P<0.01).The the level of tissue factor and the concentration of IL-2 with different concentration of CsA decreased compare with the positive control (P<0.01). 【Conclusions】Stimulant endothelial cells and abnormal expresssion of tissue factor were the key in GVHD.So the lymphocyte of donar stimulated the endothelial cells of recepter to increase the level of tissue factor was the committed step in the generation and progression of GVHD.PartⅡA Preliminary Study on the Immunological Regulation Effect of Sappan Wood's Water Extractives about Endothelial Cells and Tissue Factor in Graft-versus-host Disease【Objective】Detected the expression of tissue factor in human endothelial cell and the density of IL-2 in the cultured supernatant after mixed cultrued human endothelial cells with allogeneic lymphocyte in the diversity of Sappan Wood's water extractives and CsA.Through the detection we could get a preliminary outcome about the significance of the immunological regulation effect of Sappan Wood and the possibility which Sappan Wood substitute or part substitute the CsA in GVHD.【Methods】Human endothelial cells and allogeneic lymphocyte mixed cultrued in vitro with different CsA and Sappan Wood for 4-6 hours.Then we detected the expression of TF mRNA and the concentration of IL-2 .We could compare the results of several groups.These were detected by RT-PCR and ELISA.【Results】After human endothelial cells and allogeneic lymphocyte mixed cultrued in vitro with different CsA and Sappan Wood for 4-6 hours .We found the expression of TF mRNA and the concentration of IL-2 decreased along with the the concentration of CsA and Sappan Wood increased.And the immunological regulation effect of Sappan Wood had no significant difference with CsA on some concentration .【Conclusions】Stimulant endothelial cells and abnormal expresssion of tissue factor were the key in GVHD. The immunological regulation effect of Sappan Wood had no significant difference with CsA.It could substitute or part substitute the CsA on the treatment about GVHD.