A Study On The Possibility Of Mouse Mesenchymal Stem Cells Induced Into Corporal Smooth Muscle Cells

Objectives: To study whether the mouse mesenchymal stem cells (MSCs) have the possibility to be induced into corporal smooth muscle cells when cultured with corporal smooth muscle cell extract in vitro.Methods: The MSCs were isolated from the marrow of the 4-5 weeks old mice's femurs and tibias by gradient centrifugation and adherence to the culture plastic and were cultured in conditioned medium. After two passages, MSCs were cocultured with corporal smooth muscle cell extract at the different ratios. After 24 hours, the morphology change of MSCs were observed and the expression ofα-actin in cytoplasm was detected by immunohistochemistry.Results: Immunohistochemistry staining was positive againstα-actin on MSCs in all the cocultured group. In contrast, noα-actin expressions on MSCs were observed in the control culture group. And there is significant difference between the cocultured groups.Conclusion: Microenvironment of corporal smooth muscle cell extract can induce the differentiation of MSCs into corporal smooth muscle cell in vitro.