Expression And Significance Of Matrix Metalloproteinase-3 And It Tissue Inhibitors Of Metalloproteinase-1 In Intestinal Adhesive Tissues

Objective:To study the clinical significance and correlation of matrix metalloproteinase-3(MMP-3) and tissue inhibitor of matrix metalloproteinase-1(TIMP-1) in intestinal adhesion.Methods:Seventy SD male rats were randomly allocated to three groups,B group was 30 rats with postoperative intestinal adhesion,A group was controL,C group was normal.Ten experimental rats were sacrificed on the 2^nd,7^th and 14^th day after injection,respectively.Take adhesive tissue of small intestine respectively,the expression of MMP-3 and TIMP-1 in adhesive tissues were determined by immunohistochemistry assay and image analysis.Result:1.The expression oMMP-3 and TIMP-1 in adhesive tissues on the 2 day after surgery were significantly higher than those of normal group(P＜0.01 );MMP-3 contents in adhesive tissues on the 7day after surgery were significantly lower than the 2day after surgery(P＜0.01 );but the peak time of TIMP-1 contents was on the 7 day after surgery;MMP-3 contents in adhesive tissues continued to lower on the 14day(P＜0.01),TIMP-1 contents descended just a little(P＜0.05),but both of their contents had been basic stability already.Compare to the group of 7day after surgery and that of 2day after surgery,there was significant difference.2.Each time point model group compared with the control group were significantly different.3.In the control group,each time point comparison,no significant difference.Conclusions:The different expression of MMP-3 and TIMP-1 in different period after operation might play a role in intestinal adhesion formation.The proportional imbalance between them is closely related to the formation of intestinal adhesion.