| Objective To evaluate the association of PAHs and human gastric cancer, a method of extracting and detecting polycyclic aromatic hydrocarbons (PAHs) in human gastric cancer was established. This may contribute to explore the chemical and pathogenic factors of gastric cancer, and to provide theoretical and experimental basis for further study of gastric cancer.Methods Solid phase extraction-high performance liquid chromatography (SPE-HPLC) method was established to extract and detect PAHs in human gastric cancer tissues. 8 gastric cancer tissues and 4 normal stomach tissues, all confirmed by pathology, were randomly recruited. Five typical PAHs in both human normal stomach and gastric cancer tissues were determined by this SPE-HPLC method.Results The experimental data showed that, the sensitivity, reiteration, retrieve rate, liner range of SPE-HPLC method were reliable.Pyrene and B(a)P in human gastric cancer(1398.8±559.6ng/g and 1249.6±494.1ng/g respectivly) were significantly higher than those in normal stomachs((t=2.300, t=2.351, P<0.05), while it showed non-significant difference with dimethylnaphthalene and phenanthrene in human gastric cancer(812.6±249.8ng/g and 805.4±215.4ng/g respectivly) and normal stomachs(t=1.068, t=0.065, P>0.05).Conclusions1. It is the first time that five PAHs were found in human stomach tissuus.2. Human gastric tissue has extremely strong ability of bioconcentrating PAHs.3. There is high association of PAHs and occurrence of human gastric cancer. PAHs may be one of the most important chemical factors that lead to human gastric cancer. So it is not difficult to give a conclusion that to decrease and avoid the intake of PAHs may have a very important significance to reduce the incidence of gastric cancer. |
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