Preventve Effect Of Adenosine Triphosphate Magnesium Chlorid On Spinal Cord Ischemia Reperfusion Injury In Rabbits

Objective:To evaluate the efects and possible mechanism of ATP-Mgcl₂ on spinal cord ischemic injury in rabbits.Methods:The model of spinal cord ischemia-reperfusion in rabbits was established according to the description of Tetik.30 rabbits were randomly divided into sham operation group(A) ischemia-reperfusion group(B) and ATP-Mgcl₂ group(C).In group B and C,the infrarenal aorta was clamped for 40min followed by 48 hours reperfusion.ATP-Mgcl₂(4ml / kg·h) was injected 10min before clamping in group C,and 0.9%Saline was used as a control in the other groups.The plasma concentrations of Maleic Dialdehyde(MDA), Superoxide Dismutase(SOD),were assayed before clamping(C₀),before unclamping (C₄₀),and at 1 hour(R₁) after unclamping.After operation,the neurologic out comes were evaluated by blind method.The morphology of the spinal cord was observed.Results:(1)The concentrations of MDA after ischemia-reperfusion in group B were increased significantly compared with those before clamping and those in group A(P＜0.01),which in group C were lower significantly than those in group B(P＜0.05),but not in group A.(2)The activity of SOD after ischemia-reperfusion was lower significantly in group B than that before clamping and that in group A(P＜0.01),which was higher significantly in group C than that in group B(P＜0.05),but no in group A.(3)The ratio of paralysis in group C was lower significantly than that in group B with a high neurologic score(P＜0.01).(4)In group B,more pathological changes and a greater number of neuronaldeath were seen in group B,which was only occasionally in group C.Conclusion:The results suggest that ATP-Mgcl₂ may reduce spinal cord injury and preserve neurological function in transient spinal cord ischemia in rabbits.