Character Research On Cells Captured From Spatium Intermusculare By Porous Material Implanted In Vivo

Currently, most of the research on stem cells has focused on either embryonic or adult stem cells. From a therapeutic point of view, embryonic stem cells are the most versatile when used for the regeneration or repair of different tissues because of their multipotentiality. In addition, stem cell transplantation has shed the light to the treatment of a number of serious diseases. However, these cells must be used in the form of allografts unless therapeutic cloning techniques are utilized to generate autologous embryonic stem cells to overcome immune rejection. Because of the ethical controversies, the application of embryonic stem cells in clinical treatment are affected and hindered.Recently, Kazutoshi and colleagues have shown that induced pluripotent stem cells can be generated from adult human dermal fibroblasts and other somatic cells by retroviral transduction of four transcription factors namely Oct3/4, Sox2, Klf4, and c-Myc that are induced pluripotent stem cells in mouse. These retroviral DNA constructs could be introduced into human cells to induce human induced pluripotent stem cells. However, the concern with safety issue may become problematic in the application of this technology in clinic.A great number of studies have proven that adult stem cells have the strong flexibility or"plasticity", and can trans-differentiate into different types of tissue special cells. The characterized adult stem cells can be used for autologous transplantation. Adult stem cells may play an important role in the field of regenerative medicine in the future.Muscle could serve as a good source of collecting adult stem cells due to its vast mass and extensive network of capillaries. Muscle-derived stem cells (MDSC) are defined here as stem cells that normally reside in and can be isolated from skeletal muscles. Recent reports showed that adult skeletal muscle stem cells have the potential to differentiate into endothelial lineage, hematopoietic cells, and other cells. It suggests that MDSCs contain pluripotent stem cells.ObjectiveCurrent methods of collecting MDSCs from muscles are characterized by removing large mass of muscle from the body, minced and chopped, isolated and cultured in vitro. This method is complicated and often causes tissue damage. We try to generate a new simple method of collecting MDSCs in vivo.MethodWe first demonstrated that 3-D porous material implanted into the spatium intermusculare could capture cells. Then we confirm whether capturing cells exhibited stem cell properties (CD34, Sca-1, CD105,GFAP, AFP), the exclusive hematopoietic lineage marker CD45. Whether the capturing cells can differentiate into hematopoietic lineages in vivo; proliferate in lethally irradiated mouse vitro.ResultsWe discovered that a great number of cells migrated into the 3-D porous material implanted in vivo. Furthermore, the capturing cells exhibited stem cell properties (CD34+, Sca-1+, CD105+ , GFAP+, AFP+) and were hematogeous (CD45+). The ability of capturing cells to undergo differentiation into hematopoietic lineages was tested with methylcellulose medium. The ability of capturing cells to proliferate was confirmed in lethally irradiated mouse vitro.ConclusionWe generated a simple method by implanting 3-D porous material into spatium intermusculare to collect stem cells that does not make serious tissue destruction and thus may promote the autologous stem cell transplantation in clinic. We believe that this method might be useful to capture adult stem cells from other tissues and will have a potential application of autologous stem cell transplantation in clinic.