The Expressions Of LKN-1, CCR1 And CCR3 In Hypertensive Disorder Complicating Pregnancy

Objective Hypertensive disorder complicating pregnancy plays an important part in the raising of the morbidity and mortality of gravida and fetus.And its pathogenesis is still unclear now.It's presumed that pathophysiological change is the origin of eclampsism-eclampsia.The immune and inflammatory reaction etc.induce the spasmus of systemic arterioles,which causes the damage of systemic vascular endothelial cells,then provokes a serial of clinic symptoms.Phlogogenic factors and anti-inflammatory factors existing in the placenta such as cytokines,chemokines, adhesion molecules,signal transduction factors,growth factors and their receptors maybe take part in the pathogenesis.Chemokines are a group of polypeptides which play a great role in chemotactic and activatory responses of leukocytes.According to the construction features of their first class peptide chains,they are divided into four subfamilies,such as CXC,CC,C and CX3C.It's showed that chemokines are related to inflammatory changes in placental vessels,and such as IL-8,RANTES,MCP-1 et al.are confirmed to go up in placental vasal diseases and promote the progression of inflammation.Leukotactin-1(LKN-1)belongs to the CC subfamily,and through its receptors(CCR1 and CCR3)it can induce the chemotactic response of human endothelial cells,promote the differentiation of endothelial cells and induce the formation of vessels.And in atherosclerotic plaque its expression is obviously elevated.As we see,acute atherosclerosis takes place in the vessels of placental villus and helicine arteries of the hypertensive disorder complicating pregnancy patients, thus we presume the pathogenesis of hypertensive disorder complicating pregnancy is related to the expression of LKN-1 in placental vessels.Here we detected the expression of LKN-1 and CCR1,CCR3 in the placenta of hypertensive disorder complicating pregnancy patients to investigate the role of LKN-1,CCR1 and CCR3 in the pathogenesis of hypertensive disorder complicating pregnancy.Methods The level of LKN-1,CCR1 and CCR3 in placenta was detected from 32 patients with hypertensive disorder complicating pregnancy(hypertensive disorder complicating pregnancy group)and 32 nomal pregnant women(normal control group) by western blot(WB)and the expression of LKN-1,CCR1 and CCR3 in the two groups was detected by immunohistochemistry(IHC).Results 1.Western blot showed LKN-1 was negative in the placentas from the two groups;while CCR1 from hypertensive disorder complicating pregnancy group was significantly lower than that in normal control group(0.6608±0.0582 vs 0.8806±0.0324)(P＜0.01);and CCR3 from hypertensive disorder complicating pregnancy group was significantly lower than that in normal control group(0.1695±0.0146 vs 0.2725±0.0057)(P＜0.01).2.IHC showed LKN-1 was negative in the placentas from the two groups;CCR1 was expressed in the stromal cells and the vascular endothelial cells in both groups, and weak signal was found associated with the trophoblast layer;while CCR3 was expressed in the trophoblast cells and the vascular endothelial cells in both groups.Conclusion LKN-1 was negative in the placentas from the two groups.The down regulation of CCR1 and CCR3 was related to the occurrence and development of hypertensive disorder complicating pregnancy.