SLT Screening Of Anticancer Active Site And Researching Of Lung Cancer A549 Cell Proliferation Inhibition Mechanism

Obsjective: In this study we investigated SLT (Solanum Lyratum Thunb) screening of anticancer active site, effect on apoptosis-inducing and proliferation inhibition of tumor cell in vivo and vitro.The result can be used to establish the experimental basis for its clinical application and anticancer treatment.Methods: Active site C2,C3,D3,D5,D8 were extracted from Dried whole herbs of SLT. The human lung cancer A549 cells and H22 hepatic cancer cells is cultured in vitro;the two significant active site was selected out for experiment medicine by MTT assay.After being treated with SLT active site , morphological changes of apoptosis were observed with invert microscope;Detect cell apoptosis rate, DNA ladder was examined by DNA agarose gel electrophoresis.The expression of bid mRNA ,caspase-9 mRNA were detected by semi-quantitive RT-PCR .The animal model was developed by hypo injecting H22 hepatic cancer cells into Balb/c mice and carries on the SLT active site to the Balb/c mouse transplant lump function preliminary discussion . on five groups C3 and C2 of SLT active site was intratumorally injected into Balb/c mice in 7 day. calculated rate of tumor growth inhibition, the tumor organization pathological section was inspected, With anti-CD34 multi-clonal antibody demonstration blood vessel endothelial cell ,we carries on the artificial counting and the quantitative analysis to MVD (microvessel density) by immunhistochemical method .Results: SLT active site has the strong inhibitory action to the cancer cell multiplication, the inhibitory effect is C3>C2>D8> D5>D3 in turn. In the SLT medicine group there is obvious cell karyopyknosis, the chromatin agglutination and margination, and so on characteristics, DNA ladder is obviously on agarose gel electrophoresis. the rate of apoptosis Was significantly higher, bid mRNA, caspase-9 mRNA expression was up-regulated.On 13 Balb/c mouse hypodermic transplantation tumor appears obviously , the SLT medicine group mouse tumor is slow-growing, the pathology presents the fixity or the liquescency necrosis changes, the MVD artificial counting is lower than the blank control group. Conclusion: SLT active site C3, C2, D3,D5,D8 have the strong inhibitory action to the human lung cancer A549 cell multiplication, C3 active site is strongest inhibitory action. SLT active site C3, C2 not can only up-regulate the human lung cancer A549 cell caspase-9 and the bid gene expression and induce apoptosis of the lung cancer A549 cell, but also inhibit Balb/c mouse tumor angiogenesis to achieve the anti-tumor the function, and the C3 effect surpasses C2.