The Research Of The Affects Of Chronic Idiopathic Thrombocytopenic Purpura Mouse Model Bcl-2,PLT In Peripheral Blood By Zidian Shengyu Pulvis

Objective:To investigate the mechanism by which Zidian Shengyu Pulvis(ZSP)affects the development of CITP through observing changes of PLT number and the peripheral blood expression of Bcl-2 in CITP model mice.Method:1.The mice were divided into six groups:normal,model,hormone control,low dose of ZSP,middle dose of ZSP,and high dose of ZSP.2.The chronic CITP model was established by intraperitoneally injection of anti-BALB/c platelet serum(APS).3.The platelet number in mice was calculated by an automatic blood cell counter.The peripheral blood expression of Bcl-2 in mice was measured by ELISA.The mice in treatment groups were gavaged with ZSP for 14 consecutive days starting 8 hours after the 1st abdominal cavity injection of APS.Results:The PLT number in mice of the model group was lower than that in normal group,and the expression of Bcl-2 in mice of the model group was statistically higher than that in the normal group(P＜0.05).Compared with the model group,mice in all the treatment groups showed statistically higher PLT numbers and lowet Bcl-2 expression levels(P＜0.05).No significant difference was observed among middle dose of ZSP group,high dose of ZSP group and the hormone group.Conelusion:Bcl-2 wase expressed in peripheral blood at highet level in the CITP model mice.ZSP has functions of lowering peripheral blood expression of Bcl-2 and increasing PLT number.It plays a role in the treatment of CITP by regulating immunological functions to reduce PLT destruction.