Effbct Of Silencing PAR1 By RNA Interference On Invasive Potential Of Human Colon Cancer Cell Lines

Objective: To study the effect of RNA interference (RNAi) on PAR1 expression and the invasive petential of human colon cancer cell lines. Methods: Chemically synthesizeddouble stranded RNA (dsRNA) targeting PAR1 was transfected into human colon cancer cell Lovo using SiPORT Lipid. The transfection eficiency was observed under fluorescence confocal microscopy. Expression of PAR1 mRNA and protein was detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Cell penetrate matrigel capacity were determined by invitro experiment. Results: PAR1-siRNA effectively inhibited PAR1 mRNA and protion expression (P <0.05), and degraded invasion capability of Lovo cells (P<0.01). Conclusion: PAR1-siRNA inhibits PAR1 mRNA and protein expression, degrades invasion of human colon cancer cell.