The Research Of The Correlation Between MMP-7, MMP-9 And Abdominal Cavity Metastasis In Human Colorectal Carcinoma Using Tissue Microarray

Objective:To observe the expressions o f Matrix Metalloproteinase 7 , Matrix Metalloproteinase 9 in human Colorectal Carcinoma,and explore the correlation between MMP-7,MMP-9 and abdominal cavity metastasis in humanColorectal Carcinoma using tissue microarray. The aim of these studies is to provide further rationale basis for MMPs family members in diagnostic study of prediction and monitoring of metastasis correlated with Colorectal Carcinoma and in remedial study of anti- Colorectal Carcinoma drugs. The other aim of these studies is to explore the application of Microarray immunohistochernical technique in the detection of the index correlated with Colorectal Carcinoma.Colorectal carcinoma (CRC) is one of the most common malignancies in digestive tracts. Although comprehensive therapies have been used, the prognosis of CRC is still poor, which is possibly due to the lack of early effective diagnosis and effective targets of anti-metastasis. Therefore, further exploring the alterations of key genes related to development and progression of CRC will be considered useful.Degradating almost all components of extracellular matrix, matrix metalloproteinase-7 (MMP-7) and matrix metalloproteinase-9 (MMP-9) are hence considered to be related to invasion/metastasis of malignancies.Tissue chip, also called tissue microarray (TMA), can sample up to 1000 cylindrical tissue specimens on one glass slide. In 1998, Kononen first described this high-throughput technique and constructed a breast cancer tissue microarray. Six gene amplifications were detected by fluorescence in situ hybridization, immunohistochemistry and mRNA ISH. The results confirmed these makers were related to the prognosis of breast cancer and agreed with published results. TissueMicroarrays provide a high-throughout molecularte chnique for pathology.An umber of diffrerent applications of tissue microarrays have been described in recent publications. Because a large number of nearly identical sections can be obtained from a single MTTB, these sections could be useful for large scale interlaboratory quality control studies. Tissue microarrays can be used for educational purposes as well as to improve quality control and standardization of staining methods and interpretation. Tissue microarrays have become one of the most promising tools for the molecular and anatomic pathologist and will have many applications in cancer research, as well as in other fields of pathology.In order to investigate the impact of MMP-7 and MMP-9 on development and progression of CRC, we first constructed the tissue microarrays of CRC including 12 cases common intestinal mucosa, 50 cases adenocarcinoma with abdominal cavity metastasis and 48 cases without abdominal cavity metastasis, then detected MMP-7 and MMP-9 protein expressions by immunohistochemistry.Methods:This experiment includes three parts:1 Collect 110 cases of paraffin-embeddeds pecimen stored in the 4th hospital of HEBEI medical university, including 12 cases common intestinal mucosa, 50 cases adenocarcinoma with abdominal cavity metastasis and 48 cases without abdominal cavity metastasis.Then construct tissue microarrays using personaltis suear rayer.2 To evaluate the expression of MMP7, MMP9 using S-P method of IHC (immunohistochemistry).3 With SPSS10.0, to compare the differences of the expression between adenocarcinoma with abdominal cavity metastasis and without abdominal cavity metastasis; to evaluate the relationship of markers' expression and abdominal cavity metastasis in CRC.Results:1 Succeed in constructing three blocks of tissue microarray of CRC and 96 cases of data are eligible for analysis.2 The expression rate of MMP7 and MMP9 were much higher in adenocarcinoma with abdominal cavity metastasis (68%, 78%) than those without abdominal cavity metastasis (39.5%, 33.3%) (P<0.01)Conclusion: 1 Tissue microarray can provide a large scale of immunohi-stochemistry evaluation of MMP7 and MMP9 expression in CRC of in a short period with efficiency.2 The expression rate of MMP7 and MMP9 were much higher in adenocarcinoma with abdominal cavity metastasis than those without abdominal cavity metastasis. It seems that the case with high expression of MMP7 and MMP9 has the liability of abdominal cavity metastasis in CRC.