| Objiective To investigate the inhibiting effect of adeno-associated virus-mediated herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV)system on posterior capsule opacification(PCO) in rabbits and the toxicity in eye anterior segment.Methods1. 24 rabbits(24 eyes) were randomly divided into 4 groups .All rabbits were given phacoemulsification in each right eye.During operation,the group 1 and group 2 were injected 0.1ml Balenced salt solution(BSS) in the capsular bag,and the group 3 and group 4 were injected 0.1ml adeno-associated virus-mediated HSV-tk in the capsular bag . 24 hours later ,0.1ml BSS was injeceted in the ocular anterior chamber of group 1 and 3 ,and 0.1ml GCV was injected in the ocular anterior bag of group 2 and 4,once a day,for three days. In the four-week follow-up after operation,every right eye undertook the slit-lamp examination.2. Every right eye undertook corneal endothelium microscope examination in the 28 day after operation.Immunohistochemical staining was used to detected proliferating cell nuclear antigen(PCNA) masculine expression in posterior capsule and histological Influence of anterior segment of eye. Resoults1. slit-lamp examination:The PCO in the eyes of group 4 was markedly lower than that of other three groups(P<0.05). No statistical difference was found among the other three groups.2.Histological examination:There was no obviously inflammatory reaction and histological damage to the cornea and iris in every group.Proliferation of the lens epithelial cells in group 4 was significantly lower than that of other three groups.3.There was no statistically difference among each group on corneal endothelial cell density,mean cell area and coefficient of variation .4.PCNA masculine expression in posterior capsule in group 4 was significantly lower than that of other three groups. Conclusion adeno-associated virus-mediated HSV-tk/GCV system can effectively prevent of PCO.And there was no histological damage to the anterior segment of eye. |
PDF Full Text Request