Study On The Extraction Technology Of Sanqi And In Vitro Release Mode Of Target Ingredient In Sustained-release Preparation

Purpose To seek for better Sanqi medicinal material which is fit to experiment, establish the extraction technology of total saponinses and determination method, reveal the release behavior in vitro of 4 saponinses of Sanqi in Compound Danshen Sustained-Release Tablet, illustrate the release mode of 4 saponinses, realize well-balanced release of target ingredient.Method1. 4 saponinses (notoginsenoside R1,ginsenoside Rg1,ginsenoside Re and ginsenoside Rb1) in Sanqi are chosen as target ingredients, and the quanlity of Sanq is from Guangxi and Yunnan is evaluated by qualitative of TLC and HPLC and by quantitative of HPLC, and finally the paper choose the better medicinal material.2. The paper choose the concent of 4 saponinses and the stem plaster rate as evaluation standed, and use single-factor test in determining the better level of Reflux method,percolation method and ultrasonic extraction method, and use orthogonal test in determining the best extraction condition of 3 method above, and finally choose the best extraction technology.3. The paper chooses the concent of 4 saponinses as evaluation standed, and use HPLC in determining the dissolution sample in different time point in Compound Danshen Sustained-releaseTablet and common Compound Danshen Tablet, and finally determines the release mode.Result1. Reference substance and sample appear the same colour dot in the same Rf; The chromatography fingerprints of Sanqi from two different habits have 9 major peaks shared, and the peak area of Guangxi is relatively bigger.,and the proportion of peak area of 4 saponinses(Guangxi : Yunnan) are relatively 153.0%,117.6%,90.4% and 129.9%。2. The best extraction technology of Sanqi total saponinses is as follows: the volume, Concentration and time of the soaking are relatively 2 times of sanqi,solvent 1 and 1h;the Concentration of solvent 2 is 95%,the volume of ethanol is 10,the times is 2 and the extrcation time is 1h.;The average extraction rate of 4 saponinses is 92.47% and the average stem plaster rate is 20.20%.3. The result show that: 4 saponins of Sanqi in Compound Danshen Sustained-release Preparation have a well-balanced release behavior in 1h to 10h with Higuchi release model; 4 saponins of Sanqi in common Compound Danshen Tablet release completely in 40 min.Conclusion1. The paper choose Guangxi as habit resource of Sanqi medical material in Compound Danshen Sustained-Release Tablet;2. The extraction technology of Sanqi total saponinses can be used in pilot test and industrial production, and a better repeatability are all fit for the standed of Compound Danshen Sustained-release Preparation.3. The paper use HPLC in determining the 4 saponinses concent of Compound Danshen Sustained-release Tablet and common Compound Danshen Tablet for the first time, and provide effective determining method for quanlity control.4. 4 saponins of Sanqi in Compound Danshen Sustained-release Preparation have a well-balanced release behavior in 1h to 10h, and realize the purpose of well-balanced release.