Study On Rat Model With Polycystic Ovary Syndrome For Exercise Intervention

Objective:We established rat model with polycystic ovary syndrome for exercise intervention and observed the changes of serum hormone and ovarian morphology during this processes.Method:The fifty of female Wistar rats at 21 days of age were randomly divided into experimental group(n=25)and control group(n=25).The experimental rats were subcutaneously injected daily with testosterone propionate of 1mg/100g body weight dissolved in tea oil,while control rats were injected with tea oil.The body fat index,ovarian weight,fasting blood glucose and serum insulin(FINS),serum estrogen(E₂),serum progesterone(P),serum testosterone(T)and ovarian morphology were analyzed respectively at 7,14,21,up to 28 days during the experiments.Result:At 28 days after injection of testosterone propionate,the levels of INS, E₂ and T in serum increased significantly and ratios of FBG/FINS decreased significantly,compared with control group.The pathology of ovarian showed remarkably changes of multiple follicular cysts and significant increases in the ratios of follicular cysts to normal follicle after injection of testosterone propionate.The levels of serum E₂ increased significantly after 7 days with injection of testosterone propionate.There were no remarkably changes in serum insulin until 28 days after injection of testosterone propionate in experimental group.Ovarian morphology showed a relative thinned granulosa cell layer and a thickened theca cell layer at 7 and 14 days after injection of testosterone propionate.The amounts of atretic follicles and preantral follicles significantly increased respectively at 21and 28 days after injection of testosterone propionate, compared to controls.Conclusion:The rat model with PCOS induced by injection of testosterone propionate for 28 days are characteristic of hyperinsulinemia,abnormal sex hormone and multiple follicular cysts,which keeps on stability for long times.The rat model with PCOS can be used to study exercise intervention.