Effect Of Arsenic Trioxide On Inducing Apoptosis Of A549 Cells And Gene Expression Of Survivin And Cyclin D1

Objective : To investigat the effect of arsenic trioxide on cell proliferation,cell cycle, cell apoptosis and survivin,cyclin D1 gene expression in lung adenocarcinoma A549 cells. Methods: After A549 were treated with arsenic trioxide in different doses and with different time, MTT was used to detect the proliferation of A549 cells. The Annexin V-FITC /PI double staining analysis by flow cytometry was used to evaluate apoptosis rate. Cell cycle was elaluated by PI staining FCM anlysis. Survivin ,cyclin D1 gene expression was detected by RT-PCR and immunohistochemesitry. Results: Arsenic trioxide could inhibit cell proliferation, induce cell apoptosis and stop cell cycle at G1/S transition in A549 cells. The apoptotic rate was both dose depedent and time dependent. Survivin and cyclin D1 gene expression was down regulated in arsenic trioxide group compared with the control. Conclusion: Arsenic trioxide can effectively inhibit cell proliferatioin and induce apoptosis in lung adenocarcinoma A549 cells,this may result from its actioin of reduce survivin and cyclin D1 gene expression. Arsenic trioxide stop cell cycle at G1 phase in lung adenocarcinoma A549 cells, this may result from its actioin of reduce cyclin D1 gene expression.